High throughput screening to determine the antibacterial activity of Terminalia ferdinandiana (Kakadu plum): A proof of concept

Saleha Akter; Yasmina Sultanbawa; Daniel Cozzolino

doi:10.1016/j.mimet.2021.106169

High throughput screening to determine the antibacterial activity of Terminalia ferdinandiana (Kakadu plum): A proof of concept

J Microbiol Methods. 2021 Mar:182:106169. doi: 10.1016/j.mimet.2021.106169. Epub 2021 Feb 15.

Authors

Saleha Akter¹, Yasmina Sultanbawa², Daniel Cozzolino³

Affiliations

¹ Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Health and Food Science Precinct, Kessels Rd, Coopers Plains, QLD 4108, Queensland, Australia.
² Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Health and Food Science Precinct, Kessels Rd, Coopers Plains, QLD 4108, Queensland, Australia; ARC Industrial Transformation Training Centre for Uniquely Australian Foods, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Health and Food Science Precinct, Kessels Rd, Coopers Plains, QLD 4108, Queensland, Australia.
³ Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Health and Food Science Precinct, Kessels Rd, Coopers Plains, QLD 4108, Queensland, Australia; ARC Industrial Transformation Training Centre for Uniquely Australian Foods, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Health and Food Science Precinct, Kessels Rd, Coopers Plains, QLD 4108, Queensland, Australia.. Electronic address: d.cozzolino@uq.edu.au.

PMID: 33596404
DOI: 10.1016/j.mimet.2021.106169

Abstract

Different types of susceptibility tests are available to identify antimicrobial activity, including the disc agar diffusion and broth micro-dilution methods. In recent years, high throughput screening (HTS) methods have been considered and evaluated as an efficient method to rapidly monitor the antimicrobial potential of a wide range of plant products. The objective of this study was to test the ability of a 96-well plate reader as HTS method to evaluate the antimicrobial potential of extracts of Terminalia ferdinandiana (Kakadu plum). The main changes observed in the UV-VIS spectra of the bacteria samples were related to the biochemical and chemical compounds that might originate from the effect of the T. ferdinandiana extracts and the bacteria. Partial least squares discriminant analysis (PLS-DA) allowed the correct classification of samples according to the concentration of extract added to the culture (e.g. high, medium and low). The results of this study indicated that might be possible to record changes in the UV-VIS spectra associated with the interactions between bacteria and T. ferdinandiana extracts using a 96-well plate reader. The method was able to detect or differentiate between live and dead bacteria based on the UV-VIS spectra as a function of the addition of the T. ferdinandiana extracts.

Keywords: Antimicrobial; Chemometrics; Escherichia coli; High throughput screening (HTS); Kakadu plum; MRSA; Staphylococcus aureus; Terminalia ferdinandiana.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents* / chemistry
Anti-Bacterial Agents* / pharmacology
Bacteria / drug effects*
Fruit / chemistry*
Plant Extracts* / chemistry
Plant Extracts* / pharmacology
Proof of Concept Study
Terminalia / chemistry*

Substances

Anti-Bacterial Agents
Plant Extracts