Abstract
Fatty acid desaturase 1 (FADS1) polymorphisms alter fatty acid content in subcutaneous adipose tissue (SAT); however, existing evidence is limited and conflicting regarding the association between FADS1 variants and SAT inflammatory status. To advance this area, we conducted an exploratory study to investigate whether the common rs174537 polymorphism in FADS1 was associated with immune cell profiles in abdominal and femoral SAT in individuals with obesity. FADS1 gene expression and immune cell profiles in SAT depots were assessed by qPCR and flow cytometry, respectively. Although FADS1 gene expression was associated with genotype, no associations were observed with immune cell profiles in either depot. Our study provides additional evidence that rs174537 in FADS1 has minimal impact on inflammatory status in obese SAT.
Keywords:
Fatty acid desaturase; T cell; macrophage; white adipose tissue.
MeSH terms
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Adipose Tissue / immunology*
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Adipose Tissue / metabolism
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Adult
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Delta-5 Fatty Acid Desaturase
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Fatty Acid Desaturases / genetics*
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Fatty Acid Desaturases / immunology
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Fatty Acid Desaturases / metabolism
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Fatty Acids / metabolism
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Female
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Femur / metabolism
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Genotype
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Humans
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Intra-Abdominal Fat / immunology
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Male
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Middle Aged
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Obesity / metabolism
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Pilot Projects
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Polymorphism, Single Nucleotide / genetics
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Subcutaneous Fat / immunology
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Subcutaneous Fat / metabolism*
Substances
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Delta-5 Fatty Acid Desaturase
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Fatty Acids
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Fatty Acid Desaturases
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FADS1 protein, human
Grants and funding
C.W. is supported by a CBS-International PhD Graduate Research Assistantship and International Doctoral Tuition Scholarship from the University of Guelph. This research was supported by grants #371546 (D.M.M.) and #418323-2012RGPIN (S.S.) from National Sciences and Engineering Research Council of Canada, as well as a Canadian Innovation Foundation Grant [#225985] (S.S.). Additionally, S.S. is the recipient of a Canadian Research Chair Tier 2 – Clinical Nutrition.