Covalent capture and electrochemical quantification of pathogenic E. coli

Sarah H Klass; Laura E Sofen; Zachary F Hallberg; Tahoe A Fiala; Alexandra V Ramsey; Nicholas S Dolan; Matthew B Francis; Ariel L Furst

doi:10.1039/d0cc08420d

Covalent capture and electrochemical quantification of pathogenic E. coli

Chem Commun (Camb). 2021 Mar 9;57(20):2507-2510. doi: 10.1039/d0cc08420d.

Authors

Sarah H Klass¹, Laura E Sofen¹, Zachary F Hallberg², Tahoe A Fiala¹, Alexandra V Ramsey¹, Nicholas S Dolan¹, Matthew B Francis³, Ariel L Furst⁴

Affiliations

¹ Department of Chemistry, University of California, Berkeley, California 94720, USA.
² Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA.
³ Department of Chemistry, University of California, Berkeley, California 94720, USA and Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
⁴ Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. afurst@mit.edu.

Abstract

Pathogenic E. coli pose a significant threat to public health, as strains of this species cause both foodborne illnesses and urinary tract infections. Using a rapid bioconjugation reaction, we selectively capture E. coli at a disposable gold electrode from complex solutions and accurately quantify the pathogenic microbes using electrochemical impedance spectroscopy.

MeSH terms

Acetates / chemistry
Biosensing Techniques
Cells, Immobilized
Chlorides / chemistry
Dielectric Spectroscopy
Electrodes
Escherichia coli / chemistry*
Escherichia coli Infections / prevention & control
Foodborne Diseases / prevention & control
Gold / chemistry
Limit of Detection
Picolinic Acids / chemistry
Polyethylene Glycols / chemistry
Surface Properties

Substances

Acetates
Chlorides
Picolinic Acids
6-mercaptopicolinic acid
Polyethylene Glycols
Gold
acetyl chloride

Grants and funding

T32 GM066698/GM/NIGMS NIH HHS/United States