Genomic Analysis of an Indian G8P[1] Caprine Rotavirus-A Strain Revealing Artiodactyl and DS-1-Like Human Multispecies Reassortment

Shubhankar Sircar; Yashpal Singh Malik; Prashant Kumar; Mohd Ikram Ansari; Sudipta Bhat; S Shanmuganathan; Jobin Jose Kattoor; O R Vinodhkumar; Narayan Rishi; Nadia Touil; Souvik Ghosh; Krisztián Bányai; Kuldeep Dhama

doi:10.3389/fvets.2020.606661

Genomic Analysis of an Indian G8P[1] Caprine Rotavirus-A Strain Revealing Artiodactyl and DS-1-Like Human Multispecies Reassortment

Front Vet Sci. 2021 Jan 27:7:606661. doi: 10.3389/fvets.2020.606661. eCollection 2020.

Authors

Affiliations

¹ Division of Biological Standardization, Indian Council of Agricultural Research-Indian Veterinary Research Institute, Bareilly, India.
² Amity Institute of Virology and Immunology, J-3 Block, Amity University, Noida, India.
³ College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
⁴ Animal Disease Diagnsotic Laboratory, Purdue University, West Lafayette, IN, United States.
⁵ Division of Epidemiology, Indian Council of Agricultural Research-Indian Veterinary Research Institute, Bareilly, India.
⁶ Laboratoire de Recherche et de Biosécurité, Hôpital Militaire d'instruction Med V de Rabat, Rabat, Morocco.
⁷ Department of Biomedical Sciences, One Health Center for Zoonoses and Tropical Veterinary Medicine, Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis.
⁸ Centre for Agricultural Research, Institute for Veterinary Medical Research, Hungarian Academy of Sciences, Budapest, Hungary.
⁹ Division of Pathology, Indian Council of Agricultural Research-Indian Veterinary Research Institute, Bareilly, India.

Abstract

The surveillance studies for the presence of caprine rotavirus A (RVA) are limited in India, and the data for the whole-genome analysis of the caprine RVA is not available. This study describes the whole-genome-based analysis of a caprine rotavirus A strain, RVA/Goat-wt/IND/K-98/2015, from a goat kid in India. The genomic analysis revealed that the caprine RVA strain K-98, possess artiodactyl-like and DS-1 human-like genome constellation G8P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3. The three structural genes (VP2, VP4, and VP7) were close to caprine host having nucleotide-based identity range between 97.5 and 98.9%. Apart from them, other gene segments showed similarity with either bovine or human like genes, ultimately pointing toward a common evolutionary origin having an artiodactyl-type backbone of strain K-98. Phylogenetically, the various genes of the current study isolate also clustered inside clades comprising Human-Bovine-Caprine isolates from worldwide. The current findings add to the knowledge on caprine rotaviruses and might play a substantial role in designing future vaccines or different alternative strategies combating such infections having public health significance. To the best of our knowledge, this is the first report on the whole-genome characterization of a caprine RVA G8P[1] strain from India. Concerning the complex nature of the K-98 genome, whole-genome analyses of more numbers of RVA strains from different parts of the country are needed to comprehend the genomic nature and genetic diversity among caprine RVA.

Keywords: G8P[1] strain; India; goat; reassortment; rotavirus-A; whole-genome analysis.