Lack of association between VEGF -2578C/A polymorphism and risk of colorectal cancer in an Iranian population

Sanaz Savabkar; Neda Zali; Mahrooyeh Hadizadeh; Shabnam Tavangarroosta; Chris Young; Fateme Shojaeian; Nastaran Ebrahimi; Maziar Ashrafian Bonab; Hamid Rezvani; Farzaneh Shalileh; Ehsan Nazemalhosseini-Mojarad

Lack of association between VEGF -2578C/A polymorphism and risk of colorectal cancer in an Iranian population

Gastroenterol Hepatol Bed Bench. 2020 Winter;13(Suppl1):S47-S52.

Affiliations

¹ Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Centre, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
² School of Medicine, University of Sunderland, City Campus, Chester Road, Sunderland, SR1 3SD, UK.
³ School of Allied Health Sciences, Faculty of Health and Life Sciences, De Montfort University, Leicester, LE1 9BH, UK.
⁴ Gastroenterology and Liver Diseases Research center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

PMID: 33585003
PMCID: PMC7881395

Abstract

Aim: Here, we evaluated the VEGF gene -2578C/A polymorphism as a potential susceptibility factor in colorectal cancer (CRC) occurrence amongst Iranian CRC patients.

Background: Vascular endothelial growth factor (VEGF) is a key regulatory factor in angiogenesis which plays essential roles in the development of malignancy in colorectal cancer (CRC), as the third most prevalent cancer worldwide.

Methods: VEGF -2578C/A polymorphism was evaluated in 200 CRC patients and 200 healthy control subjects via restriction fragment length polymorphism analysis.

Results: The frequencies of CC, AC and AA genotypes among CRC patients were 22.5%, 51% and 26.5%, respectively, with their respective genotype frequencies at 16%, 54% and 30% in control cohorts (P=0.247). The A allele frequency among the case group was 52% and for control group, it was 57%. C allele frequency in case and control groups was 48% and 43%, respectively (p=0.156). No significant association was observed (p=0.990) between this polymorphism and CRC stage.

Conclusion: Our findings provide limited support for the hypothesis that the -2578C/A VEGF are associated with increased risk of colorectal cancer in Iranian colorectal cancer patients and suggest instead that meta data studies, which have previously relied upon populations definitions such as 'Asian', should more specifically take into account country of origin when associating prognostic value to a given genotype.

Keywords: Angiogenesis; Colorectal cancer; Single nucleotide polymorphism; VEGF; Vascular endothelial growth factor.