Detection of bacterial colonization by the spectral changes of surface-enhanced Raman reporters

Abstract

In times of widespread multiple antibiotic resistance, the bacterial colonization of crucial medical surfaces should be detected as fast as possible. In this work, we present the non-destructive SERS method for the detection of bacterial colonization. SERS is an excellent tool for the monitoring of suitable substances in low concentrations. The SERS substrate was prepared by the aggregation of citrate-stabilized gold nanoparticles and the adsorption of the reporters (crystal violet, thiamine, and adenine). We have tested the substrate for the detection of clinically relevant S. aureus and P. aeruginosa bacteria. The SERS spectra before and after the substrate incubation revealed the degradation of the reporter by the growing bacteria. The growth of P. aeruginosa was detected using the substrates with preadsorbed crystal violet or adenine. The suitable reporter for the detection of S. aureus remains to be discovered. The selection of the reporters resistant to exposure but easily degraded by bacteria will open the way for the in situ monitoring of bacterial colonization, thus complementing the arsenal of methods in the battle against hospital infections.

Keywords: Au nanoparticles; Biosensor; P. aeruginosa; S. aureus; SERS.