Development and neutralization analysis of recombinant BVDVs expressing a CSF single chain antibody in vitro

Jie Tao; Benqiang Li; Jinghua Cheng; Ying Shi; Xiaohui Shen; Huili Liu

doi:10.1016/j.biologicals.2021.01.004

Development and neutralization analysis of recombinant BVDVs expressing a CSF single chain antibody in vitro

Biologicals. 2021 Apr:70:38-43. doi: 10.1016/j.biologicals.2021.01.004. Epub 2021 Feb 10.

Authors

Jie Tao¹, Benqiang Li¹, Jinghua Cheng¹, Ying Shi¹, Xiaohui Shen², Huili Liu³

Affiliations

¹ Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China; Shanghai Key Laboratory of Agricultural Genetic Breeding, Shanghai, 201106, China; Shanghai Engineering Research Center of Pig Breeding, Shanghai, 201302, China.
² Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China.
³ Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China; Shanghai Key Laboratory of Agricultural Genetic Breeding, Shanghai, 201106, China; Shanghai Engineering Research Center of Pig Breeding, Shanghai, 201302, China. Electronic address: huilil@163.com.

PMID: 33582026
DOI: 10.1016/j.biologicals.2021.01.004

Abstract

Although the immunization against swine fever (SF) is compulsory in China, it has still emerged in several areas at times. Herein, this study was conducted to develop an antibody vaccine which can clear the classical swine fever virus (CSFV) immediately after the pathogen invasion. Bovine viral diarrhoea virus (BVDV) infectious cDNA clone pASH28 was used to express a single-chain fragment variable (scFv) antibody against CSFV (CSFV/scFv) by reverse genetic technique. CSFV/scFv was inserted at the N-terminus of the C or E^rns gene, generating two rBVDVs (rBVDV/C-CSFV/scFv and rBVDV/E^rns-CSFV/scFv). Although both the rBVDVs could stably propagate on MDBK cells, different cellular characteristics existed. Obvious green fluorescence against the CSFV/scFv antibody could be visual on the cytomembrane or outside of the cells infected with rBVDV/E^rns-CSFV/scFv, while much weaker fluorescence was observed in rBVDV/C-CSFV/scFv - infected cells. The CSFV/scFv antibodies induced by the two rBVDVs could recognize CSFV, but the rBVDV/E^rns-CSFV/scFv induced stronger viral neutralization reaction. It was speculated that the neutralization activity might be associated with the expression location of CSFV/scFv antibody. The datas in this study provide evidence that rBVDV/E^rns-CSFV/scFv may be engineered as a new antibody vaccine candidate against CSFV in the future.

Keywords: Antibody vaccine; CSFV; Neutralization; Recombinant BVDVs; Single-chain fragment variable.

MeSH terms

Animals
Antibodies, Viral / immunology*
Classical Swine Fever Virus / genetics
Classical Swine Fever Virus / immunology
Classical Swine Fever* / prevention & control
Diarrhea Viruses, Bovine Viral*
Neutralization Tests
Reverse Genetics
Single-Chain Antibodies / immunology*
Swine
Viral Envelope Proteins / immunology*
Viral Vaccines* / immunology

Substances

Antibodies, Viral
Single-Chain Antibodies
Viral Envelope Proteins
Viral Vaccines