Identification and antioxidant activity of bovine bone collagen-derived novel peptides prepared by recombinant collagenase from Bacillus cereus

Yihang Song; Yousi Fu; Shiyang Huang; Langxing Liao; Qian Wu; Yali Wang; Fuchun Ge; Baishan Fang

doi:10.1016/j.foodchem.2021.129143

Identification and antioxidant activity of bovine bone collagen-derived novel peptides prepared by recombinant collagenase from Bacillus cereus

Food Chem. 2021 Jul 1:349:129143. doi: 10.1016/j.foodchem.2021.129143. Epub 2021 Feb 1.

Authors

Yihang Song¹, Yousi Fu¹, Shiyang Huang¹, Langxing Liao², Qian Wu¹, Yali Wang¹, Fuchun Ge³, Baishan Fang⁴

Affiliations

¹ Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
² Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. Electronic address: liaolx@stu.xmu.edu.cn.
³ Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China. Electronic address: 20420142201202@stu.xmu.edu.cn.
⁴ Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; The Key Lab for Synthetic Biotechnology of Xiamen City, Xiamen University, Xiamen, Fujian 361005, China; The Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, China. Electronic address: fbs@xmu.edu.cn.

PMID: 33581432
DOI: 10.1016/j.foodchem.2021.129143

Abstract

Millions of tons of collagen-rich bovine bone are produced as byproducts of the consumption of beef. Hydrolyzing bovine bone collagen (BBC) is an effective measure for both increasing its added value and protecting the environment. In this study, a kind of recombinant bacterial collagenase mining from Bacillus cereus was successfully performed and applied to hydrolyze BBC to collagen-soluble peptides (CPP). Response surface methodology (RSM) was applied to optimize the processing conditions of antioxidant CPP, attaining a distinguished ABTS free radical scavenging activity of 99.21 ± 0.35% while keeping DPPH free radical scavenging activity and reducing power at high levels under the optimal condition. Furthermore, we identified five new antioxidant peptides by LC-MS/MS with typical collagen repeated Gly-Xaa-Yaa sequence units within the CPP. These results suggest that our recombinant collagenase is a powerful tool for degrading collagen and the CPP are promising candidates for antioxidant and related functional food applications.

Keywords: Antioxidant peptides; Bacillus cereus; Bovine bone collagen; Recombinant collagenase; Response surface methodology.

MeSH terms

Animals
Antioxidants / metabolism
Antioxidants / pharmacology
Bacillus cereus / enzymology*
Bone and Bones / chemistry*
Cattle
Collagen / chemistry*
Collagenases / metabolism*
Hydrolysis
Peptide Fragments / metabolism*
Peptide Fragments / pharmacology*
Recombinant Proteins / metabolism*

Substances

Antioxidants
Peptide Fragments
Recombinant Proteins
Collagen
Collagenases