Structure characterization and in vitro immunomodulatory activities of carboxymethyl pachymaran

Feng Liu; Ying Liu; Xi Feng; Salam A Ibrahim; Wen Huang

doi:10.1016/j.ijbiomac.2021.02.046

Structure characterization and in vitro immunomodulatory activities of carboxymethyl pachymaran

Int J Biol Macromol. 2021 May 1:178:94-103. doi: 10.1016/j.ijbiomac.2021.02.046. Epub 2021 Feb 10.

Authors

Feng Liu¹, Ying Liu¹, Xi Feng², Salam A Ibrahim³, Wen Huang⁴

Affiliations

¹ College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
² Department of Nutrition, Food Science and Packaging, San Jose State University, San Jose, CA 95192, USA.
³ Department of Family and Consumer Sciences, North Carolina A&T State University, 171 Carver Hall, Greensboro, NC 27411, United States.
⁴ College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: huangwen@mail.hzau.edu.cn.

PMID: 33577815
DOI: 10.1016/j.ijbiomac.2021.02.046

Abstract

Carboxymethyl pachymaran (CMP) was prepared from Poria cocos polysaccharide by carboxymethylation. Two types of CMP (CMP-1 and CMP-2) were further purified by DEAE-52 anion-exchange chromatography. The structure characteristics and immunomodulatory activities of CMP-1 and CMP-2 were investigated. CMP-1 was determined as β-(1 → 3)-d-glucan. A β-(1 → 3)-d-glucan backbone structure was also found in CMP-2, which was mainly consistent of mannose and glucose, with the mole ratio of 0.03:1. The molecular weight of CMP-1 was 126.1 kDa with a 30.4 nm irregular sphere in distilled water. However, the molecular weight of CMP-2 was 172.6 kDa in a 19.9 nm spherical structure in water solution. Both CMP-1 and CMP-2 had triple helical structure, which can promote the proliferation and the phagocytosis of macrophages. Moreover, CMP-1 and CMP-2 both could improve the secretions of NO, TNF-α and IL-6 by increasing the expression of iNOS, TNF-α and IL-6 mRNA, but CMP-1 exhibited a stronger immunomodulatory ability than that of CMP-2. Our results indicated that CMP-1 and CMP-2 can act as potential immunomodulatory agents.

Keywords: Carboxymethyl pachymaran; Chain conformation; Immunomodulatory; Structure.

MeSH terms

Animals
Cell Proliferation
Cytokines
Glucans / chemistry*
Immunomodulation
Macrophages / drug effects*
Mice
Molecular Structure
Molecular Weight
Phagocytosis
RAW 264.7 Cells
Wolfiporia / chemistry*

Substances

Cytokines
Glucans
carboxymethyl pachyman
pachymaran