Assigning Function to Phylogeny: MAR-FISH

Jeppe L Nielsen

doi:10.1007/978-1-0716-1115-9_14

Assigning Function to Phylogeny: MAR-FISH

Methods Mol Biol. 2021:2246:225-236. doi: 10.1007/978-1-0716-1115-9_14.

Author

Jeppe L Nielsen¹

Affiliation

¹ Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark. jln@bio.aau.dk.

PMID: 33576992
DOI: 10.1007/978-1-0716-1115-9_14

Abstract

Microautoradiography (MAR) is a technique by which assimilated radioactive tracers incorporated into the biomass can be detected by a film emulsion. This allows for the testing of cellular preferences in electron donors and acceptors of individual cells in complex microbial assemblages, as well as the ability to take up substrates under diverse environmental exposures.Combination with staining techniques such as fluorescence in situ hybridization (FISH) can be used to identify the involved cells. Here, the practical aspects of a combined microautoradiography and fluorescence in situ hybridization (MAR-FISH) approach are described.

Keywords: Ecophysiology; MAR-FISH; Microautoradiography; Microbial ecology; Single cell imaging.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autoradiography / methods*
Biomass
Electrons
In Situ Hybridization, Fluorescence / methods*
Microbiota / genetics
Phylogeny