LncRNA ELFN1-AS1 Promotes Retinoblastoma Growth and Invasion via Regulating miR-4270/SBK1 Axis

Wanguo Feng; Ruixi Zhu; Junlong Ma; Han Song

doi:10.2147/CMAR.S281536

LncRNA ELFN1-AS1 Promotes Retinoblastoma Growth and Invasion via Regulating miR-4270/SBK1 Axis

Cancer Manag Res. 2021 Feb 5:13:1067-1073. doi: 10.2147/CMAR.S281536. eCollection 2021.

Authors

Wanguo Feng¹, Ruixi Zhu², Junlong Ma³, Han Song²

Affiliations

¹ Department of Refractive Surgery, Dalian Aier Eye Hospital, Dalian, 116092, People's Republic of China.
² Department of Ophthalmology, Heilongjiang Provincial Hospital, Harbin Institute of Technology, Harbin, 150036, People's Republic of China.
³ Department of Ophthalmology, Dalian University Affiliated Xinhua Hospital, Dalian, 116021, People's Republic of China.

Abstract

Background: Long noncoding RNA (lncRNA) has been reported to play important roles in tumor initiation. However, how lncRNA ELFN1-AS1 affects retinoblastoma development remains unclear. Thus, we sought to elucidate its functions in retinoblastoma progression.

Methods: ELFN1-AS1 expression was measured in retinoblastoma tissues and normal tissues by qRT-PCR. CCK8, colony formation and Transwell assay were carried out to investigate the effects of ELFN1-AS1 knockdown on cell malignant behaviors. Bioinformatics analyses were performed to predict the relationship among ELFN1-AS1, miR-4270 and SBK1.

Results: ELFN1-AS1 was highly expressed in retinoblastoma tissues and cell lines. ELFN1-AS1 was positively correlated with retinoblastoma progression and prognosis. ELFN1-AS1 knockdown curtailed retinoblastoma proliferation, migration and invasion. ELFN1-AS1 was the competing endogenous RNA for miR-4270 and promoted SBK1expression.

Conclusion: Altogether, our findings demonstrated that ELFN1-AS1 promotes retinoblastoma progression through mediating miR-4270/SBK1 axis and might be a promising therapeutic target.

Keywords: ELFN1-AS1; SBK1; miR-4270; progression; retinoblastoma.