Real-Time Tracking of Highly Luminescent Mesoporous Silica Particles Modified with Europium β-Diketone Chelates in Living Cells

Jong-Seok Kim; Sung Ki Lee; Hansol Doh; Myeong Yun Kim; Do Kyung Kim

doi:10.3390/nano11020343

Real-Time Tracking of Highly Luminescent Mesoporous Silica Particles Modified with Europium β-Diketone Chelates in Living Cells

Nanomaterials (Basel). 2021 Jan 29;11(2):343. doi: 10.3390/nano11020343.

Authors

Jong-Seok Kim¹, Sung Ki Lee², Hansol Doh³, Myeong Yun Kim⁴, Do Kyung Kim⁴

Affiliations

¹ Myunggok Medical Research Institute, College of Medicine, Konyang University, Daejeon 35365, Korea.
² Department of Obstetrics and Gynecology, Konyang University Hospital, Daejeon 35365, Korea.
³ Department of Food Science and Technology, University of California-Davis, Davis, CA 95616, USA.
⁴ Department of Anatomy, College of Medicine, Konyang University Hospital, Daejeon 35365, Korea.

Abstract

Highly luminescent europium complexes modified mesoporous silica particles (MSP) were synthesized as an imaging probes for both in-vitro diagnostic and in-vivo cellular tracking agents. Europium β-diketone chelates (4,4,4-trifluoro-l-(2-thienyl)-l,3-butanedione) trioctylphosphine europium (III) (Eu(TTA)₃(P(Oct)₃)₃) were incorporated inside the nanocavities that existed in hierarchical MSP (Eu@MSP). The MSP and Eu@MSP on mouse bone marrow-derived macrophages (BMDMs) did not show any toxic effect. The MSP and Eu@MSP in the BMDMs were found at cytoplasm without any degradation and immunogenicity. However, both pro- and anti-inflammatory cytokines of macrophages were significantly increased when lipopolysaccharide and a high concentration (100 μg/mL) of MSP and Eu@MSP were treated simultaneously.

Keywords: beta-diketone; europium complex; inflammation; live cell image; mesoporous silica.

Abstract

Grants and funding