Increasing the Production of Volatile Fatty Acids from Corn Stover Using Bioaugmentation of a Mixed Rumen Culture with Homoacetogenic Bacteria

Nanditha Murali; Keerthi Srinivas; Birgitte K Ahring

doi:10.3390/microorganisms9020337

Increasing the Production of Volatile Fatty Acids from Corn Stover Using Bioaugmentation of a Mixed Rumen Culture with Homoacetogenic Bacteria

Microorganisms. 2021 Feb 8;9(2):337. doi: 10.3390/microorganisms9020337.

Authors

Nanditha Murali^{1

2}, Keerthi Srinivas^{1

2}, Birgitte K Ahring^{1

2

3}

Affiliations

¹ Department of Chemical Engineering, Voiland College of Engineering and Architecture, Washington State University, Pullman, WA 99163, USA.
² Bio-Products, Sciences and Engineering Laboratory, Washington State University, Richland, WA 99354, USA.
³ Department of Biological Systems Engineering, Washington State University, Pullman, WA 99163, USA.

Abstract

Volatile fatty acids (VFA) are industrially versatile chemicals and have a major market. Although currently produced from petrochemicals, chemical industries are moving towards more bio-based VFA produced from abundant, cheap and renewable sources such as lignocellulosic biomass. In this study, we examined the effect of bioaugmentation with homoacetogenic bacteria for increasing VFA production in lignocellulose fermentation process. The central hypothesis of this study was that inhibition of methanogenesis in an in vitro rumen bioreactor fed with lignocellulosic biomass hydrolysate increases the hydrogen partial pressure, which can be redirected towards increased VFA production, particularly acetic acid, through targeted bioaugmentation with known homoacetogenic bacteria. In this study, methanogenesis during ruminal fermentation of wet exploded corn stover was initially inhibited with 10 mM of 2-bromoethanesulfonate (BES), followed by bioaugmentation with either Acetitomaculum ruminis and Acetobacterium woodii in two separate bioreactors. During the inhibition phase, we found that addition of BES decreased the acetic acid yield by 24%, while increasing headspace hydrogen from 1% to 60%. After bioaugmentation, the headspace hydrogen was consumed in both bioreactors and the concentration of acetic acids increased 45% when A. ruminis was added and 70% with A. woodii added. This paper demonstrates that mixed microbial fermentation can be manipulated to increase VFA production through bioaugmentation.

Keywords: Acetobacterium woodii; acetic acid; bioaugmentation; homoacetogens; rumen.