Lipid and carotenoid production by the Rhodosporidium toruloides mutant in cane molasses

Xuehai Zheng; Rongfei Hu; Duo Chen; Jiannan Chen; Wenjin He; Luqiang Huang; Chentao Lin; Huibin Chen; Youqiang Chen; Jinmao Zhu; Feng Qi; Ting Xue

doi:10.1016/j.biortech.2021.124816

Lipid and carotenoid production by the Rhodosporidium toruloides mutant in cane molasses

Bioresour Technol. 2021 Apr:326:124816. doi: 10.1016/j.biortech.2021.124816. Epub 2021 Feb 3.

Authors

Xuehai Zheng¹, Rongfei Hu¹, Duo Chen¹, Jiannan Chen¹, Wenjin He¹, Luqiang Huang¹, Chentao Lin¹, Huibin Chen¹, Youqiang Chen¹, Jinmao Zhu¹, Feng Qi¹, Ting Xue²

Affiliations

¹ The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, Southern Institute of Oceanography, College of Life Sciences, Fujian Normal University, Fuzhou, China.
² The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, Southern Institute of Oceanography, College of Life Sciences, Fujian Normal University, Fuzhou, China. Electronic address: xueting@fjnu.edu.cn.

PMID: 33563516
DOI: 10.1016/j.biortech.2021.124816

Abstract

Cane molasses is beneficial for lipid and carotenoid production in microalgae. We made a survey for the lipid and carotenoid production profile of R. toruloides M18 (MT) with various concentrations of molasses under nitrogen-deficited conditions. The production of α-linolenate and torularhodin from MT were 1.22- and 14.68-fold higher than those of the wild-type strain. We observed that molasses at concentrations of 35 g/L and 70 g/L represented a cheap and environmentally friendly strategy for producing lipids and carotenoids. Transcriptome and WGCNA analysis demonstrated that the genes relevant to the lipid and carotenoid production, including MYB, bHLH, Δ-4 desaturase, Δ-12 desaturase and FA2H, were significantly highly expressed. The results indicated that molasses could represent an inexpensive means for achieving high lipids and carotenoids production in R. toruloides.

Keywords: Cane molasses; Lipid; Rhodosporidium toruloides; Torularhodin; Torulene; β-carotene.

MeSH terms

Basidiomycota*
Canes
Carotenoids
Lipids
Molasses*
Rhodotorula

Substances

Lipids
Carotenoids

Supplementary concepts

Rhodotorula toruloides