Chlorella vulgaris cultivation in simulated wastewater for the biomass production, nutrients removal and CO2 fixation simultaneously

Abstract

Chlorella vulgaris (C. vulgaris) was promising microalgae to simultaneously achieve biomass production, carbon dioxide (CO₂) fixation, nutrients removal and proteins production especially under different conditions of CO₂ gas and wastewaters. Results presented that maximal specific growth rate of C. vulgaris was 0.21-0.35 d^-1 and 0.33-0.43 d^-1 at 0.038% and 10% CO₂ respectively, and corresponding maximal CO₂ fixation rate was attended with 4.51-14.26 and 56.26-85.72 mg CO₂·L^-1·d^-1. C. vulgaris showed good wastewater removal efficiency of nitrogen and phosphorus at 10% CO₂ with 96.12%-99.61% removal rates. Nitrogen fixation amount achieved 41.86 mg L^-1 when the initial NH₄Cl concentration was set at 60 mg L^-1 at 10% CO₂. Improved total protein (25.01-365.49 mg) and amino acids (24.56-196.44 mg) contents of C. vulgaris biomass was observed with the increasing of added CO₂ and ammonium concentrations. Moreover, the developed kinetic function of C. vulgaris growth depends on both phosphorus quota and nitrogen quota with correlation coefficient (R²) ranged from 0.68 to 0.97. Computed maximal consumed nutrients concentrations (ΔC_max) based on Logistic function are positively related to initial NH₄⁺-N concentrations, which indicated that adding ammonium could stimulate the utilization of both phosphorus and nitrogen.

Keywords: Carbon dioxide fixation; Chlorella vulgaris; Kinetics; Nitrogen; Phosphorus.