Construction and biological characterization of an infectious full-length cDNA clone of a Chinese isolate of Wheat yellow mosaic virus

Fan Zhang; Shuang Liu; Tianye Zhang; Zhuangxin Ye; Xiaolei Han; Kaili Zhong; Jian Yang; Jianping Chen; Peng Liu

doi:10.1016/j.virol.2021.01.018

Construction and biological characterization of an infectious full-length cDNA clone of a Chinese isolate of Wheat yellow mosaic virus

Virology. 2021 Apr:556:101-109. doi: 10.1016/j.virol.2021.01.018. Epub 2021 Feb 5.

Authors

Fan Zhang¹, Shuang Liu², Tianye Zhang³, Zhuangxin Ye⁴, Xiaolei Han⁵, Kaili Zhong⁶, Jian Yang⁷, Jianping Chen⁸, Peng Liu⁹

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant Virology, Ningbo University, Ningbo, 315211, China. Electronic address: zf950418@163.com.
² State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant Virology, Ningbo University, Ningbo, 315211, China. Electronic address: 22016090@zju.edu.cn.
³ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant Virology, Ningbo University, Ningbo, 315211, China. Electronic address: Ztye1995@163.com.
⁴ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant Virology, Ningbo University, Ningbo, 315211, China. Electronic address: yzx244522794@163.com.
⁵ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant Virology, Ningbo University, Ningbo, 315211, China. Electronic address: hanxiaolei123@yeah.net.
⁶ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant Virology, Ningbo University, Ningbo, 315211, China. Electronic address: zhongkaili@nbu.edu.cn.
⁷ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant Virology, Ningbo University, Ningbo, 315211, China. Electronic address: nather2008@163.com.
⁸ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant Virology, Ningbo University, Ningbo, 315211, China. Electronic address: jpchen2001@126.com.
⁹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Institute of Plant Virology, Ningbo University, Ningbo, 315211, China. Electronic address: wood319@126.com.

PMID: 33561697
DOI: 10.1016/j.virol.2021.01.018

Abstract

Wheat yellow mosaic virus (family Potyviridae; genus Bymovirus), is an important soil-borne virus that causes serious economic losses in wheat. In this study, we constructed infectious cDNA clones of WYMV genomic RNAs under the control of 35S or SP6 promoter for versatile usage (agroinfiltration or in vitro RNA transcription). Our results showed that an Agrobacterium-mediated inoculation system enabled WYMV to infect the leaves of Nicotiana benthamiana without causing WYMV systemic infection. However, in vitro transcripts from infectious cDNA clones using the SP6 promoter promoted WYMV systemic infection of wheat plants, which was then developed for further assays. The optimal temperature for virus multiplication and systemic infection of wheat was 8 °C. Additionally, a synergistic effect between WYMV and Chinese wheat mosaic virus (CWMV) was also detected. This is the first report of the construction of a Chinese isolate of WYMV and should facilitate the investigation of viral pathogenesis.

Keywords: Bymovirus; Infectious clones; Systemic infection; WYMV; Wheat.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Plant Diseases / virology*
Plant Viruses* / genetics
Plant Viruses* / isolation & purification
Plant Viruses* / pathogenicity
Triticum / virology*

Supplementary concepts

Chinese wheat mosaic virus