Analysis the alteration of systemic inflammation in old and young APP/PS1 mouse

Liangyun Liu; Jianing Cao; Chujun Huang; Erdong Yuan; Jiaoyan Ren

doi:10.1016/j.exger.2021.111274

Analysis the alteration of systemic inflammation in old and young APP/PS1 mouse

Exp Gerontol. 2021 May:147:111274. doi: 10.1016/j.exger.2021.111274. Epub 2021 Feb 6.

Authors

Liangyun Liu¹, Jianing Cao², Chujun Huang³, Erdong Yuan³, Jiaoyan Ren⁴

Affiliations

¹ School of Food Science and Engineering, South China University of Technology, Guangzhou, China. Electronic address: 201820124839@mail.scut.edu.cn.
² School of Food Science and Engineering, South China University of Technology, Guangzhou, China. Electronic address: 201820247706@mail.scut.edu.cn.
³ School of Food Science and Engineering, South China University of Technology, Guangzhou, China.
⁴ School of Food Science and Engineering, South China University of Technology, Guangzhou, China. Electronic address: jyren@scut.edu.cn.

PMID: 33561502
DOI: 10.1016/j.exger.2021.111274

Abstract

The impairment of cognitive function was considered as a major clinic feature in Alzheimer's disease (AD) patients. Thus, a number of researches related to AD were focused on the changes in brain. However, as a neurodegenerative disorder with systemic inflammation, the periphery organs may also play a key role in AD pathology. Here, we pose the hypothesis that histopathology and inflammatory response of periphery organs may alter with aging in APP/PS1 mouse model. Therefore, we performed immunohistochemical staining technology to double label Aβ plaques and microglia cells in brain. The H&E staining was performed in periphery tissues and the mRNA expression of inflammatory factors IL-6, IL-10 and TNF-α were also determined. Next, the index of oxidative stress was measured. Consequently, the level of inflammatory factors was significantly increased in 24 months APP/PS1 mice. Furthermore, the enzyme activity of SOD, CAT and GSH were significantly decreased in colon and other organs. Our results demonstrated the increased inflammation response and declined antioxidative capacity of periphery organs in aged APP/PS1 mice, which suggesting that a more comprehensive perspective to study AD were necessary.

Keywords: Alzheimer's disease (AD); Inflammation; Oxidative index; Periphery organs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Alzheimer Disease* / genetics
Amyloid beta-Peptides
Amyloid beta-Protein Precursor* / genetics
Animals
Disease Models, Animal
Humans
Inflammation
Mice
Mice, Inbred C57BL
Mice, Transgenic
Presenilin-1 / genetics

Substances

Amyloid beta-Peptides
Amyloid beta-Protein Precursor
Presenilin-1