Engineering 3D genome organization

Haifeng Wang; Mengting Han; Lei S Qi

doi:10.1038/s41576-020-00325-5

Engineering 3D genome organization

Nat Rev Genet. 2021 Jun;22(6):343-360. doi: 10.1038/s41576-020-00325-5. Epub 2021 Feb 8.

Authors

Haifeng Wang^#¹, Mengting Han^#¹, Lei S Qi^{2

3

4}

Affiliations

¹ Department of Bioengineering, Stanford University, Stanford, CA, USA.
² Department of Bioengineering, Stanford University, Stanford, CA, USA. stanley.qi@stanford.edu.
³ Department of Chemical and Systems Biology, Stanford University, Stanford, CA, USA. stanley.qi@stanford.edu.
⁴ ChEM-H Institute, Stanford University, Stanford, CA, USA. stanley.qi@stanford.edu.

^# Contributed equally.

PMID: 33558716
DOI: 10.1038/s41576-020-00325-5

Abstract

Cancers and developmental disorders are associated with alterations in the 3D genome architecture in space and time (the fourth dimension). Mammalian 3D genome organization is complex and dynamic and plays an essential role in regulating gene expression and cellular function. To study the causal relationship between genome function and its spatio-temporal organization in the nucleus, new technologies for engineering and manipulating the 3D organization of the genome have been developed. In particular, CRISPR-Cas technologies allow programmable manipulation at specific genomic loci, enabling unparalleled opportunities in this emerging field of 3D genome engineering. We review advances in mammalian 3D genome engineering with a focus on recent manipulative technologies using CRISPR-Cas and related technologies.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
CRISPR-Cas Systems*
Cell Nucleus / genetics*
Gene Editing*
Genetic Engineering / methods*
Genome*
Genomics / methods*
Humans

Abstract

Publication types

MeSH terms

Grants and funding