Abstract
We developed a cDNA TRAP display for the rapid selection of antibody-like proteins in various conditions. By modifying the original puromycin linker in the TRAP display, a monobody was covalently attached to the cDNA. As a proof-of-concept, we demonstrated a rapid model selection of an anti-EGFR1 monobody in a solution containing ribonuclease.
MeSH terms
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Antibodies, Monoclonal / chemistry*
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Biosensing Techniques
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DNA, Complementary / chemistry*
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Humans
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Peptide Library
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Protein Binding
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Puromycin / chemistry
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RNA, Messenger / chemistry
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Ribonucleases / chemistry
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Sensitivity and Specificity
Substances
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Antibodies, Monoclonal
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DNA, Complementary
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Peptide Library
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RNA, Messenger
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Puromycin
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Ribonucleases