Cold atmospheric plasma increases IBRV titer in MDBK cells by orchestrating the host cell network

Yujie Miao; Peiyu Han; Dong Hua; Renwu Zhou; Zhengbing Guan; Qing Lv; Xiaofeng Dai

doi:10.1080/21505594.2021.1883933

Cold atmospheric plasma increases IBRV titer in MDBK cells by orchestrating the host cell network

Virulence. 2021 Dec;12(1):679-689. doi: 10.1080/21505594.2021.1883933.

Authors

Yujie Miao¹, Peiyu Han², Dong Hua³, Renwu Zhou⁴, Zhengbing Guan¹, Qing Lv³, Xiaofeng Dai²

Affiliations

¹ Wuxi School of Medicine, Jiangnan University , Wuxi, China.
² The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi, China.
³ Affiliated Hospital of Jiangnan University , Wuxi China.
⁴ School of Chemical and Biomolecular Engineering, University of Sydney , Sydney, Australia.

Abstract

Enhancing virus multiplication could assist in the rapid production of vaccines against viral diseases. Cold atmospheric plasma (CAP), a physical approach relying on reactive oxygen species to achieve the desirable cellular outcome, was shown to be effective in enhancing virus propagation, where bovine rhinotrachieitis virus and Madin-Darby Bovine Kidney cells were used as the modeling virus and cell line, respectively. CAP was shown to create synergies with virus infection in arresting host cells at the G2/M stage, decreasing cell membrane potential, increasing intracellular calcium level, and inducing selective autophagy. In addition, CAP was demonstrated to suppress virus-triggered immunogenic signaling as evaluated by IRF7 expression. We presented evidences on CAP-triggered maximization of host resources toward virus multiplication that is advantageous for viral vaccine production, and opened a novel regime for applying CAP in the sector of medical care and health.

Keywords: Cold atmospheric plasma; IBRV; MDBK; host-virus interaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Autophagy / drug effects
Cattle
Cell Cycle / drug effects
Cell Line
Host Microbial Interactions / drug effects*
Host Microbial Interactions / immunology
Kidney / cytology
Plasma Gases / pharmacology*
Virus Replication / drug effects*
Virus Replication / immunology

Substances

Plasma Gases

Grants and funding

This study was funded by the National Natural Science Foundation of China (Grant No. 81972789), the National Science and Technology Major Project (Grant No. 2018ZX10302205-004-002), Fundamental Research Funds for the Central Universities (Grant No. JUSRP22011), Technology Development Funding of Wuxi (Grant No. WX18IVJN017). These funding sources have no role in the writing of the manuscript or the decision to submit it for publication.