Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

Daniella C Terenzi; Ehab Bakbak; Justin Z Trac; Mohammad Al-Omran; Adrian Quan; Hwee Teoh; Subodh Verma; David A Hess

doi:10.1016/j.xpro.2021.100311

Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples

STAR Protoc. 2021 Feb 1;2(1):100311. doi: 10.1016/j.xpro.2021.100311. eCollection 2021 Mar 19.

Authors

Daniella C Terenzi^{1

2}, Ehab Bakbak^{1

3}, Justin Z Trac³, Mohammad Al-Omran^{2

3

4

5}, Adrian Quan¹, Hwee Teoh^{2

6}, Subodh Verma^{1

2

3

5}, David A Hess^{3

4

7

8}

Affiliations

¹ Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael's Hospital, Toronto, ON, Canada.
² Institute of Medical Science, University of Toronto, Toronto, ON, Canada.
³ Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada.
⁴ Division of Vascular Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael's Hospital, Toronto, ON, Canada.
⁵ Department of Surgery, University of Toronto, Toronto, ON, Canada.
⁶ Division of Endocrinology and Metabolism, Li Ka Shing Knowledge Institute of St. Michael's Hospital, Toronto, ON, Canada.
⁷ Molecular Medicine Research Laboratories, Robarts Research Institute, London, ON, Canada.
⁸ Department of Physiology and Pharmacology, Western University, London, ON, Canada.

Abstract

The examination of circulating pro-vascular progenitor cell frequency and function is integral in understanding aberrant blood vessel homeostasis in individuals with cardiometabolic disease. Here, we outline the characterization of progenitor cell subsets from peripheral blood using high aldehyde dehydrogenase (ALDH) activity, an intracellular detoxification enzyme previously associated with pro-vascular progenitor cell status. Using this protocol, cells can be examined by flow cytometry for ALDH activity and lineage restricted cell surface markers simultaneously. For complete details on the use and execution of this protocol, please refer to Terenzi et al. (2019) and Hess et al. (2019, 2020).

Keywords: Cell biology; Cell isolation; Flow cytometry/mass cytometry; Stem cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aldehyde Dehydrogenase / analysis*
Aldehyde Dehydrogenase / metabolism
Blood Cells / physiology
Cell Count / methods
Cell Differentiation
Cell Proliferation
Cells, Cultured
Flow Cytometry / methods*
Humans
Neovascularization, Physiologic
Regeneration
Stem Cells / cytology
Stem Cells / metabolism*

Substances

Aldehyde Dehydrogenase

Grants and funding

CIHR/Canada