Regional N-glycan and lipid analysis from tissues using MALDI-mass spectrometry imaging

STAR Protoc. 2021 Jan 29;2(1):100304. doi: 10.1016/j.xpro.2021.100304. eCollection 2021 Mar 19.

Abstract

N-glycans and lipids are structural metabolites that play important roles in cellular processes. Both show unique regional distribution in tissues; therefore, spatial analyses of these metabolites are crucial to our understanding of cellular physiology. Matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) is an innovative technique that enables in situ detection of analytes with spatial distribution. This workflow details a MALDI-MSI protocol for the spatial profiling of N-glycans and lipids from tissues following application of enzyme and MALDI matrix. For complete details on the use and execution of this protocol, please refer to Drake et al. (2018) and Andres et al. (2020).

Keywords: Mass spectrometry; Metabolism.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Diagnostic Imaging / methods
  • Humans
  • Lipids / analysis*
  • Lipids / chemistry
  • Polysaccharides / analysis*
  • Polysaccharides / chemistry
  • Spatial Analysis
  • Specimen Handling / methods
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • Lipids
  • Polysaccharides