Purification and preservation of satellite cells from human skeletal muscle

Katharine Striedinger; Emilie Barruet; Jason H Pomerantz

doi:10.1016/j.xpro.2021.100302

Purification and preservation of satellite cells from human skeletal muscle

STAR Protoc. 2021 Jan 29;2(1):100302. doi: 10.1016/j.xpro.2021.100302. eCollection 2021 Mar 19.

Authors

Katharine Striedinger¹, Emilie Barruet¹, Jason H Pomerantz^{1

2}

Affiliations

¹ Program in Craniofacial Biology and Department of Orofacial Sciences, University of California, San Francisco, San Francisco, CA, USA.
² Division of Plastic and Reconstructive Surgery, Department of Surgery, University of California, San Francisco, San Francisco, CA, USA.

Abstract

Regeneration and repair of skeletal muscle is driven by tissue-specific progenitor cells called satellite cells, which occupy a minority of the cells in the muscle. This protocol provides researchers with techniques to efficiently isolate and purify functional satellite cells from human muscle tissue. The proven techniques described here enable the preparation of purified and minimally altered satellite cells for in vitro and in vivo experimentation and for potential clinical applications. For complete details on the use and execution of this protocol, please refer to Barruet et al. (2020) and Garcia et al. (2018).

Keywords: Cell culture; Cell isolation; Flow cytometry/mass cytometry; Single cell.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Cell Culture Techniques / methods
Cell Separation / methods*
Flow Cytometry / methods
Humans
Muscle Fibers, Skeletal / physiology
Muscle, Skeletal / physiology
Regeneration / physiology
Satellite Cells, Skeletal Muscle / cytology*
Satellite Cells, Skeletal Muscle / physiology
Specimen Handling / methods*
Stem Cells / physiology

Abstract

Publication types

MeSH terms

Grants and funding