FeII 4L4 Tetrahedron-Assisted Three-Way Junction Probe for Multiple miRNA Detection

Chun-Yan Qu; Li-Sha Zhou; Li-Hong Shu; Qian Huang

doi:10.1021/acsomega.0c05798

Fe^II ₄L₄ Tetrahedron-Assisted Three-Way Junction Probe for Multiple miRNA Detection

ACS Omega. 2021 Jan 20;6(4):3330-3335. doi: 10.1021/acsomega.0c05798. eCollection 2021 Feb 2.

Authors

Chun-Yan Qu¹, Li-Sha Zhou¹, Li-Hong Shu¹, Qian Huang¹

Affiliation

¹ Laboratory Department, The Seventh People's Hospital of Chengdu, Chengdu 610021, China.

Abstract

MicroRNAs (miRNAs) modulate a variety of cellular signaling pathways and play a vital role in cell-to-cell communication. The overlapped expression of a certain miRNA is commonly reported to be related to cancers. Therefore, combined detection of multiple miRNAs is of great significance for cancer diagnosis. Herein, we developed a Fe^II ₄L₄ tetrahedron-assisted three-way junction (3WJ) probe, which exhibited a higher stability than the normal 3WJ probe, for multiple miRNA detection. In this method, the simultaneous existence of miRNA-21 and miRNA-144 triggers the release of the Y3 sequence in the Fe^II ₄L₄ tetrahedron-assisted 3WJ probe, which in turn triggers subsequent CRISPR-Cas12a-assisted rolling circle amplification. Based on this, simultaneous detection of miRNA-21 and miRNA-144 was achieved. Furthermore, we also applied this method to the detection of miRNAs in clinical samples and achieved good agreement with quantitative real-time polymerase chain reaction (qRT-PCR), indicating its significant potentials in early diagnosis and treatment of cancer.