Dataset on the differentiation of THP-1 monocytes to LPS inducible adherent macrophages and their capacity for NO/iNOS signaling

Adem Ozleyen; Yakup Berkay Yilmaz; Tugba Boyunegmez Tumer

doi:10.1016/j.dib.2021.106786

Dataset on the differentiation of THP-1 monocytes to LPS inducible adherent macrophages and their capacity for NO/iNOS signaling

Data Brief. 2021 Jan 21:35:106786. doi: 10.1016/j.dib.2021.106786. eCollection 2021 Apr.

Authors

Adem Ozleyen¹, Yakup Berkay Yilmaz¹, Tugba Boyunegmez Tumer²

Affiliations

¹ Graduate Program of Biomolecular Sciences, School of Graduate Studies, Çanakkale Onsekiz Mart University, 17020 Çanakkale, Turkey.
² Department of Molecular Biology and Genetics, Faculty of Science and Art, Çanakkale Onsekiz Mart University, 17020 Çanakkale, Turkey.

Abstract

When THP-1 cells are differentiated into adherent macro-phage-like cells, they respond to inflammatory stimuli by changing their phenotypes to an activation state and altering the expression of inflammation-related genes. Nitric oxide (NO) is a diatomic molecule implicating in various pathological conditions including tissue damage, ER stress, obesity, and cancer. The sustained inflammatory microenvironment leads to increased NO release through the activation of the inducible nitric oxide synthase (iNOS) gene in macrophages. Here, we provide a dataset on the optimized conditions for the THP-1 differentiation and the induction of NO/iNOS signaling under inflammatory stimulus. The human monocytic cells were differentiated into adherent macrophage-like phenotype by phorbol-12-myristate-13-acetate (PMA) stimulation under optimized conditions. In this study, NO/iNOS signaling capacity and the regulation of other pro-inflammatory genes including TNF-α, IL-1β, and COX-2 in the LPS-induced THP-1 were examined.

Keywords: Inflammation; Nitric Oxide; PMA differentiation; THP-1 monocytic cells; iNOS.