The Effect of MMP-2 Inhibitor 1 on Osteogenesis and Angiogenesis During Bone Regeneration

Liangjun Jiang; Kunkun Sheng; Cong Wang; Deting Xue; Zhijun Pan

doi:10.3389/fcell.2020.596783

The Effect of MMP-2 Inhibitor 1 on Osteogenesis and Angiogenesis During Bone Regeneration

Front Cell Dev Biol. 2021 Jan 22:8:596783. doi: 10.3389/fcell.2020.596783. eCollection 2020.

Authors

Liangjun Jiang^{1

2}, Kunkun Sheng¹, Cong Wang¹, Deting Xue^{1

2}, Zhijun Pan^{1

2}

Affiliations

¹ Department of Orthopedics, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
² Orthopedics Research Institute of Zhejiang University, Hangzhou, China.

Abstract

Bone regeneration is a popular research focus around the world. Recent studies have suggested that the formation of a vascular network as well as intrinsic osteogenic ability is important for bone regeneration. Here, we show for the first time that matrix metalloproteinase (MMP) 2 inhibitor 1 (MMP2-I1) has a positive role in the osteogenesis of human bone marrow mesenchymal stem cells (hBMSCs) and angiogenesis of human vascular endothelial cells (HUVECs). MMP2-I1 activated the p38/mitogen-activated protein kinase signaling pathway to promote the osteogenesis of hBMSCs, and promoted the angiogenesis of HUVECs via the hypoxia-inducible factor-1α signaling pathway. We also found that MMP2-I1 enhanced bone formation using a rat tibial defect model and prevented bone loss using an ovariectomy-induced mouse model of osteoporosis. Data from the mouse model demonstrated that MMP2-I1 generated more type H vessels (CD31^hiEmcn^hi) when preventing bone loss. These results provide important insights into the regulatory effects of MMP2-I1 on bone regeneration.

Keywords: MMP-2 inhibitor 1; angiogenic; bone healing; matrix metalloproteinase 2; osteogenic differentiation.