Coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, has rapidly spread and caused a severe global pandemic. Because no specific drugs are available for COVID-19 and few vaccines are available for SARS-CoV-2, accurate and rapid diagnosis of COVID-19 has been the most crucial measure to control this pandemic. Here, we developed a portable bifunctional electrical detector based on graphene fieldeffect transistors for SARS-CoV-2 through either nucleic acid hybridization or antigen-antibody protein interaction, with ultra-low limits of detection of ~0.1 and ~1 fg mL-1 in phosphate buffer saline, respectively. We validated our method by assessment of RNA extracts from the oropharyngeal swabs of ten COVID-19 patients and eight healthy subjects, and the IgM/IgG antibodies from serum specimens of six COVID-19 patients and three healthy subjects. Here we show that the diagnostic results are in excellent agreement with the findings of polymerase chain reaction-based optical methods; they also exhibit rapid detection speed (~10 min for nucleic acid detection and ~5 min for immunoassay). Therefore, our assay provides an efficient, accurate tool for high-throughput point-of-care testing.
Electronic supplementary material: Supplementary material is available in the online version of this article at 10.1007/s40843-020-1577-y.
新型冠状病毒肺炎(COVID-19)正在多个国家快速传播, 已 经导致了严重的全球大流行. 由于目前没有针对此类病人的特效 药和针对此病毒的疫苗, 准确、快速地进行新冠病人检测成为了 控制大流行最有效的措施. 本文中我们开发了一种基于石墨烯场 效应晶体管的便携式双功能电检测仪, 其通过核酸互补杂交或者 抗原-抗体特异性结合作用, 能分别进行病毒核酸序列检测和抗体 检测, 检测限分别低至0.1和1 fg mL−1. 我们通过临床样品检测进一 步评估了此方法: 从10个新冠病人和8个正常人咽拭子中提取RNA 直接用于核酸检测; 从6个新冠病人和3个正常人血清中提取抗体 用于抗体检测. 临床样品检测结果和基于聚合酶链反应的光学方 法结果完全吻合, 同时此方法拥有超快的检测速度(核酸检测需 10 min, 抗体检测需5 min). 因此, 我们的实验提供了一种有效、准 确、高通量的新冠现场即时检测工具.
Keywords: COVID-19; biosensor; immunoassay; nucleic acid detection; point-of-care testing.
© Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020.