Primary biodegradation and mineralization of aryl organophosphate flame retardants by Rhodococcus-Sphingopyxis consortium

Junhuan Wang; Thet Su Hlaing; May Thet Nwe; Mar Mar Aung; Chao Ren; Wei Wu; Yanchun Yan

doi:10.1016/j.jhazmat.2021.125238

Primary biodegradation and mineralization of aryl organophosphate flame retardants by Rhodococcus-Sphingopyxis consortium

J Hazard Mater. 2021 Jun 15:412:125238. doi: 10.1016/j.jhazmat.2021.125238. Epub 2021 Jan 26.

Authors

Junhuan Wang¹, Thet Su Hlaing², May Thet Nwe³, Mar Mar Aung⁴, Chao Ren⁵, Wei Wu⁶, Yanchun Yan⁷

Affiliations

¹ Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: 82101171004@caas.cn.
² Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China; Biotechnology Research Department, Department of Research and Innovation, Kyaukse, Myanmar. Electronic address: thetsuhlaing11@gmail.com.
³ Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China; Biotechnology Research Department, Department of Research and Innovation, Kyaukse, Myanmar. Electronic address: maythetnwe1@googlemail.com.
⁴ Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China; Department of Biotechnology, Mandalay Technological University, Mandalay, Myanmar. Electronic address: marmaraung1@gmail.com.
⁵ Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: 379477084@qq.com.
⁶ Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: wuwei02@caas.cn.
⁷ Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: yanyanchun@caas.cn.

PMID: 33550123
DOI: 10.1016/j.jhazmat.2021.125238

Abstract

In this study, the biodegradation towards aryl organophosphate flame retardants (aryl-OPFRs) was investigated by the Rhodococcus-Sphingopyxis consortium, mixture of strain Rhodococcus sp. YC-JH2 and Sphingopyxis sp. YC-JH3. The optimal ratio between the two composition strains was determined as 1:1. Under the optimum condition (pH 8, 35 °C and 0% salinity), the consortium could utilize aryl-OPFRs as sole carbon source and degrade them rapidly with half-life of 4.53, 21.11 and 23.0 h for triphenyl phosphate (TPhP), tricresyl phosphate (TCrP) and 2-ethylhexyl diphenyl phosphate (EHDPP) respectively. The consortium maintained high degrading efficiency under a wide of range of pH (6-10), temperature (20-40 °C) and salinity (0-6%). Besides, the consortium could rapidly degrade high concentration of TPhP and no inhibitory effect towards degradation speed was observed up to 500 mg/L. The effect of metal ions and surfactants was estimated. Most metal ions exhibited significant inhibition, except Zn²⁺ and Pb²⁺, which showed no effect or slight promotion. Ionic surfactants could severely reduce the degrading capacity, while nonionic surfactants showed no effect. With abundant inoculation of the consortium, mineralization higher than 75% could be achieved within a week. This study provides efficient microorganisms for bioremediation of aryl-OPFRs contamination.

Keywords: Biodegradation; Mineralization; Organophosphate flame retardants; Rhodococcus-Sphingopyxis consortium; Triphenyl phosphate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biodegradation, Environmental
Flame Retardants*
Organophosphates
Rhodococcus*
Sphingomonadaceae*

Substances

Flame Retardants
Organophosphates