The potential of the oleaginous yeast Rhodotorula paludigena CM33 to produce biolipids

Chotika Gosalawit; Sumeth Imsoonthornruksa; Brandon H Gilroyed; Lucas Mcnea; Apichat Boontawan; Mariena Ketudat-Cairns

doi:10.1016/j.jbiotec.2021.01.021

The potential of the oleaginous yeast Rhodotorula paludigena CM33 to produce biolipids

J Biotechnol. 2021 Mar 10:329:56-64. doi: 10.1016/j.jbiotec.2021.01.021. Epub 2021 Feb 4.

Authors

Chotika Gosalawit¹, Sumeth Imsoonthornruksa², Brandon H Gilroyed³, Lucas Mcnea⁴, Apichat Boontawan⁵, Mariena Ketudat-Cairns⁶

Affiliations

¹ School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Suranaree Sub-district, Muang District, Nakhon Ratchasima, 30000, Thailand. Electronic address: gosarawit@hotmail.com.
² School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Suranaree Sub-district, Muang District, Nakhon Ratchasima, 30000, Thailand. Electronic address: tonikuya@hotmail.com.
³ School of Environmental Sciences, University of Guelph Ridgetown Campus, 120 Main Street East, Ridgetown, Ontario, N0P 2C0, Canada. Electronic address: bgilroye@uoguelph.ca.
⁴ School of Environmental Sciences, University of Guelph Ridgetown Campus, 120 Main Street East, Ridgetown, Ontario, N0P 2C0, Canada. Electronic address: lmcnea01@uoguelph.ca.
⁵ School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Suranaree Sub-district, Muang District, Nakhon Ratchasima, 30000, Thailand. Electronic address: apichat@sut.ac.th.
⁶ School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Suranaree Sub-district, Muang District, Nakhon Ratchasima, 30000, Thailand. Electronic address: ketudat@sut.ac.th.

PMID: 33549673
DOI: 10.1016/j.jbiotec.2021.01.021

Abstract

Sixty-seven yeast strains were isolated from castor beans then their endogenous lipids were stained by Nile Red (NR) fluorescence dye, and flow cytometry was used to obtain a strain with a high relative mean fluorescence intensity (MFI) value. The highest MFI value was obtained for strain CM33, which produced a maximum lipid content of 20.8 % dry cell weight (DCW). Based on the sequence of the ITS-5.8S-ITS rDNA and D1/D2 26S rDNA regions, CM33 showed 99 % identity with Rhodotorula paludigena. The potential of CM33 to assimilate various carbon sources was examined by growth on minimal media using glucose, glycerol, sucrose or xylose. CM33 was grown in glucose-based medium for 96 h and exhibited a maximum lipid content of 23.9 % DCW. Furthermore, when cells were cultured on molasses waste, their biomass, lipid content and lipid concentration reached 16.5 g/L, 37.1 % DCW and 6.1 g/L, respectively. These results demonstrated the potential of R. paludigena CM33 to contribute to a value-added carbon chain by converting renewable waste materials for biolipid production.

Keywords: Flow cytometry; Lipid metabolism; Molasses; Nile Red fluorescence; Oleaginous yeast; Rhodotorula sp..

MeSH terms

Biomass
Lipids
Rhodotorula* / genetics
Yeasts

Substances

Lipids

Supplementary concepts

Rhodotorula paludigena