In-Situ Recovery of Persipeptides from Streptomyces zagrosensis Fermentation Broth by Enhanced Adsorption

Hamed Kazemi Shariat Panahi; Fatemeh Mohammadipanah; Farzaneh Rahmati; Aliakbar Tarlani; Javad Hamedi

doi:10.30498/IJB.2020.125909.2231

In-Situ Recovery of Persipeptides from Streptomyces zagrosensis Fermentation Broth by Enhanced Adsorption

Iran J Biotechnol. 2020 Apr 1;18(2):e2231. doi: 10.30498/IJB.2020.125909.2231. eCollection 2020 Apr.

Authors

Hamed Kazemi Shariat Panahi^{1

2}, Fatemeh Mohammadipanah¹, Farzaneh Rahmati¹, Aliakbar Tarlani³, Javad Hamedi¹

Affiliations

¹ Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran.
² Faculty of Medicine and Health Sciences, Macquarie University, NSW, Australia.
³ Chemistry and Chemical Engineering Research Center of Iran (CCERCI), Pazhouhesh Blvd., 17th Km of Tehran-Karaj Highway,1496813151 Tehran, Iran.

Abstract

Background: Drug discovery process is growing considerably due to the noteworthy resource of natural products. Persipeptides A and B are cyclopeptide antibiotics, which are produced by Streptomyces zagrosensis UTMC 1154. Although extraction of culture broth with the help of solvent has been optimized previously, no effort for In-Situ extraction of persipeptides has been done yet.

Objective: To produce a high quantity of persipeptides for further drug evaluation, it is crucial to design approaches aimed at improvement of the extraction yield.

Materials and methods: Amberlite XAD-16N was employed into the fermentation culture medium of S. zagrosensis in order to enhance the In-Situ extraction of persipeptides. Effects of resin content (%), resin addition time (h), and fermentation time (day) were investigated by a two-level full factorial experimental design.

Results: The main factors of resin content (%) and the interaction of resin content (%) with resin addition time (day) were found to be important using ANOVA. The results showed the amount of 0.33 % (w.v^-1) amberlite XAD-16N added at 27.2 h post-inoculation was the most effective combination to increase the efficiency of In-Situ adsorption capacity of persipeptides.

Conclusions: The provided method requires 3.3 g resin and 200 mL methanol for the extraction of persipeptides from each liter of fermentation culture of S. zagrosensis in less than 15 min. Apart from cost-efficiently and simplicity, this procedure enhanced the recovery of persipeptides by 7 % and 3 times, compared to ISP2 medium without any resin after 4 and 7 days of fermentation, respectively. Therefore, this method can be regarded as a cost-efficient enhancement approach for the production of these newly-discovered metabolites before implementing the genetic manipulation or intensive media optimization, demanding considerable time and effort.

Keywords: Amberlite Resin; Experimental design; Full factorial optimization; In situ metabolic extraction; Natural product; Persipeptides.