m6AmPred: Identifying RNA N6, 2'-O-dimethyladenosine (m6Am) sites based on sequence-derived information

Abstract

N6,2'-O-dimethyladenosine (m⁶A_m) is a reversible modification widely occurred on varied RNA molecules. The biological function of m⁶A_m is yet to be known though recent studies have revealed its influences in cellular mRNA fate. Precise identification of m⁶A_m sites on RNA is vital for the understanding of its biological functions. We present here m6AmPred, the first web server for in silico identification of m⁶A_m sites from the primary sequences of RNA. Built upon the eXtreme Gradient Boosting with Dart algorithm (XgbDart) and EIIP-PseEIIP encoding scheme, m6AmPred achieved promising prediction performance with the AUCs greater than 0.954 when tested by 10-fold cross-validation and independent testing datasets. To critically test and validate the performance of m6AmPred, the experimentally verified m⁶A_m sites from two data sources were cross-validated. The m6AmPred web server is freely accessible at: https://www.xjtlu.edu.cn/biologicalsciences/m6am, and it should make a useful tool for the researchers who are interested in N6,2'-O-dimethyladenosine RNA modification.

Keywords: EIIP-PseEIIP; Feature analysis; N6,2′-O-dimethyladenosine (m(6)A(m)); Sequence-derived features; eXtreme Gradient Boosting (XgbDart).