Human cytomegalovirus is present in the odontogenic epithelium of ameloblastoma

Mohammed Amjed Alsaegh; Sudhir Rama Varma; Alaa Muayad Altaie; Shengrong Zhu

doi:10.1080/20002297.2021.1874699

Human cytomegalovirus is present in the odontogenic epithelium of ameloblastoma

J Oral Microbiol. 2021 Jan 19;13(1):1874699. doi: 10.1080/20002297.2021.1874699.

Authors

Mohammed Amjed Alsaegh^{1

2}, Sudhir Rama Varma³, Alaa Muayad Altaie⁴, Shengrong Zhu²

Affiliations

¹ Department of Oral and Craniofacial Health Sciences, College of Dental Medicine, University of Sharjah, Sharjah, UAE.
² Department of Oral and Maxillofacial Surgery, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, P.R. China.
³ Department of Clinical Sciences, College of Dentistry, Ajman University, Ajman, UAE.
⁴ Sharjah Medical Research Institute, Medical College, Sharjah University, Sharjah, UAE.

Abstract

Background: The factor behind the activation of the remnant odontogenic tissues and development of odontogenic cysts and tumors is poorly understood.This study aimed to investigate the presence of human cytomegalovirus (HCMV) in dentigerous cyst (DC), odontogenic keratocyst (OKC), and ameloblastoma (AB). Methods: The study included 41 samples, which distributed into DC (n=13), OKC (n=12), and AB (n=16). Conventional PCR assay and IHC analysis were used to detect the HCMV-DNA and HCMV glycoprotein B (HCMV-gB) respectively. Results: HCMV-DNA was detected in 10 samples (62.5%) of AB, four samples (30.8%) of DC, and three samples (25 %) of OKC respectively (χ² test = 1.195, p= 0.247). Meanwhile, HCMV-gB was found in 12 (75%) of AB, in 2 (15.4%) of DC, and absent in OKC (0.0%) (χ² test = 4.122, p= 0.042). Conclusions: The high prevalence of HCMV inside the odontogenic epithelium of AB could indicate a possible role of the virus in the oncogenesis and/or oncomodulation of the AB. Additionally, we recommend the IHC for the detection of HCMV in the odontogenic tumors like AB.

Keywords: HCMV; ameloblastoma; cytomegalovirus; dentigerous cyst; keratocyst; odontogenic; odontogenic tumor.