Generation and assessment of high-quality mouse oocytes and embryos following nicotinamide mononucleotide administration

Yilong Miao; Jingyue Chen; Qian Gao; Bo Xiong

doi:10.1016/j.xpro.2021.100298

Generation and assessment of high-quality mouse oocytes and embryos following nicotinamide mononucleotide administration

STAR Protoc. 2021 Jan 22;2(1):100298. doi: 10.1016/j.xpro.2021.100298. eCollection 2021 Mar 19.

Authors

Yilong Miao¹, Jingyue Chen¹, Qian Gao², Bo Xiong¹

Affiliations

¹ College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
² College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.

Abstract

The low quality of oocytes is one of the main causes of the suboptimal reproductive outcome of female mammals with advanced maternal age. Here, we present a detailed protocol to obtain high-quality oocytes and embryos from aged mice by nicotinamide mononucleotide (NMN) administration. We also describe fluorescence staining procedures to assess the organelle dynamics in oocytes, and in vitro fertilization and embryo culture systems to evaluate the influence of NMN on the fertilization ability and embryonic development potential. For complete information on the use and execution of this protocol, please refer to Miao et al. (2020).

Keywords: Cell culture; Cell isolation; Microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blastocyst / metabolism*
Embryo Culture Techniques
Embryonic Development / drug effects*
Female
Fertilization in Vitro
Mice
Nicotinamide Mononucleotide / pharmacology*
Oocytes / metabolism*

Substances

Nicotinamide Mononucleotide