Antibiofilm effect of C-10 massoia lactone toward polymicrobial oral biofilms

Diyah Tri Utami; Sylvia Utami Tunjung Pratiwi; Herman P Spaink; Tetiana Haniastuti; Triana Hertiani

doi:10.4103/japtr.JAPTR_105_20

Antibiofilm effect of C-10 massoia lactone toward polymicrobial oral biofilms

J Adv Pharm Technol Res. 2021 Jan-Mar;12(1):89-93. doi: 10.4103/japtr.JAPTR_105_20. Epub 2021 Jan 9.

Authors

Diyah Tri Utami¹, Sylvia Utami Tunjung Pratiwi^{2

3}, Herman P Spaink⁴, Tetiana Haniastuti⁵, Triana Hertiani^{2

3}

Affiliations

¹ Doctoral Program Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.
² Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.
³ Department of Centre for Natural Antiinfective Research, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia.
⁴ Department Animal Sciences and Health, Institute of Biology, Leiden University, Leiden, Netherlands.
⁵ Department of Oral Biology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Abstract

This study is aimed to test the efficacy of C-10 Massoia lactone in oral polymicrobial degradation. Polymicrobial of Streptococcus sanguinis, Streptococcus mutans, Lactobacillus acidophilus, and Actinomyces viscosus were studied. C-10 Massoia lactone against biofilm degradation was investigated using modified crystal violet for biofilm staining. The effectiveness of C-10 Massoia lactone against biofilms was calculated by the minimum biofilm inhibitory concentration (MBIC₅₀) and the minimum value of biofilm eradication concentration (MBEC₅₀). Scanning electron microscope was used to study biofilm cell viability and morphological changes. The results showed a degradation effect of C-10 Massoia lactone against mature oral polymicrobial at 0.25% v/v. C-10 Massoia lactone can degrade polymicrobial biofilms of S. mutans, S. sanguinis, L. acidophilus and A. viscosus. This compound can destroy the extracellular polymeric substances (EPS) of polymicrobial biofilms. The potential application of C-10 Massoia lactone for anti-polymicrobial medication should be applied in such a way that any negative effects are minimized. Further research is needed to confirm the findings of this study.

Keywords: Actinomyces viscosus; C10 Massoia lactone; Lactobacillus acidophilus; Streptococcus spp; polymicrobial biofilms; scanning electron microscope.