Establishment of iPSC (KRIBBi001-A) from CD34+ group O D-negative bone marrow blood

Jieun Kim; Hyebin Koh; Xing Zhen; Dong-Seok Lee; Hye-Yeong Ha; Jong-Hee Lee

doi:10.1016/j.scr.2021.102199

Establishment of iPSC (KRIBBi001-A) from CD34⁺ group O D-negative bone marrow blood

Stem Cell Res. 2021 Mar:51:102199. doi: 10.1016/j.scr.2021.102199. Epub 2021 Jan 26.

Authors

Jieun Kim¹, Hyebin Koh², Xing Zhen³, Dong-Seok Lee³, Hye-Yeong Ha⁴, Jong-Hee Lee⁵

Affiliations

¹ Futuristic Animal Resource & Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
² Futuristic Animal Resource & Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea; Department of Functional Genomics, KRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon, Republic of Korea.
³ School of Life Sciences, BK21 FOUR KNU Creative BioResearch Group, Kyungpook National University, Daegu, Republic of Korea.
⁴ Division of Intractable Disease Research, Korea National Institute of Health, Cheongju, Republic of Korea.
⁵ National Primate Research Center (NPRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea. Electronic address: jonglee@kribb.re.kr.

PMID: 33529979
DOI: 10.1016/j.scr.2021.102199

Abstract

Human induced pluripotent stem cells with indefinite propagation in vitro provide a potential donor source of cells including erythroid cells for human therapy. Since group O D-negative (RhD^-) blood cells are considered as universal donors for transfusion, it is compelling to derive iPSC line from group O/RhD^- sample as a new cellular source to generate universal RBCs. The resulting iPSC line derived from group O/RhD^- somatic source showed typical features of pluripotent stem cells and could provide an unprecedented cellular tool to develop universal therapeutics for blood transfusion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bone Marrow
Cell Differentiation
Erythroid Cells
Humans
Induced Pluripotent Stem Cells*
Pluripotent Stem Cells*