The complete mitochondrial genome of the oleaginous microalgae Ankistrodesmus falcatus strain UCP001 from the Peruvian Amazon

Marianela Cobos; Gad E Grandez; J Dylan Maddox; Carlos G Castro; Hicler N Rodríguez; Segundo L Estela; Miguel A Grández; Jae D Paredes; Rodil Tello-Espinoza; Pedro M Adrianzén; Jorge L Marapara; Juan C Castro

doi:10.1080/23802359.2020.1846470

The complete mitochondrial genome of the oleaginous microalgae Ankistrodesmus falcatus strain UCP001 from the Peruvian Amazon

Mitochondrial DNA B Resour. 2021 Jan 8;6(1):50-52. doi: 10.1080/23802359.2020.1846470.

Authors

Marianela Cobos¹, Gad E Grandez^{1

2}, J Dylan Maddox^{1

3

4}, Carlos G Castro^{1

2}, Hicler N Rodríguez^{1

2}, Segundo L Estela¹, Miguel A Grández¹, Jae D Paredes¹, Rodil Tello-Espinoza⁵, Pedro M Adrianzén^{2

6}, Jorge L Marapara^{2

6}, Juan C Castro^{2

6}

Affiliations

¹ Laboratorio de Biotecnología y Bioenergética (LBB), Universidad Científica del Perú (UCP), Loreto, Perú.
² Unidad Especializada de Biotecnología, Centro de Investigaciones de Recursos Naturales de la Amazonía (CIRNA), Universidad Nacional de la Amazonia Peruana (UNAP), Loreto, Perú.
³ Pritzker Laboratory for Molecular Systematics and Evolution, Field Museum of Natural History, Chicago, IL, USA.
⁴ Environmental Sciences, American Public University System, Charles Town, WV, USA.
⁵ Departamento Académico de Manejo Forestal y Medio Ambiente, Facultad de Ciencias Forestales, Universidad Nacional de la Amazonia Peruana (UNAP), Ciudad Universitaria - Zungarococha, Loreto, Perú.
⁶ Departamento Académico de Ciencias Biomédicas y Biotecnología, Facultad de Ciencias Biológicas, Universidad Nacional de la Amazonia Peruana (UNAP), Ciudad Universitaria - Zungarococha, Loreto, Perú.

Abstract

Ankistrodesmus falcatus strain UCP001 is a native oleaginous microalgae isolated from the Peruvian Amazon basin. In this study we sequenced, de novo assembled, and functionally annotated the complete mitochondrial genome of the native oleaginous microalgae Ankistrodesmus falcatus strain UCP001 (Accesion number MT701044). This mitogenome is a typical circular double stranded DNA molecule of 41,048 bp in total length with G + C content of 37.4%. The mitogenome contains 49 genes, including 18 protein coding genes, 5 ribosomal (rRNA) genes and 26 transfer RNA (tRNA) genes. A phylogenetic analysis of 18 microalgae species indicated that Ankistrodesmus falcatus strain UCP001 was closely related to Ourococcus multisporus and Raphidocelis subcapitata. The complete mitochondrial genome sequence of Ankistrodesmus falcatus strain UCP001 enriches genomic resources of oleaginous native microalgae from the Peruvian Amazon for further basic and applied research.

Keywords: Biofuel; chlorophyta; green algae; mitochondrial genome; molecular sequence annotation.

Grants and funding

This work was supported by Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica (FONDECYT) with grant contract No. 213-2015-FONDECYT. JDM was supported, in part, by a grant from Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica [44–2019-FONDECYT] during a portion of this research. Additionally, we thank the Laboratorio de Biotecnología y Bioenergética, Universidad Científica del Perú (UCP) and the Pritzker Laboratory for Molecular Systematics and Evolution, Field Museum of Natural History, and the Grainger Bioinformatics Center for providing necessary infrastructure and equipment.