Characteristics of Carbapenemase-Producing Klebsiella pneumoniae Isolated in the Intensive Care Unit of the Largest Tertiary Hospital in Bangladesh

Takashi Okanda; Anwarul Haque; Takuro Koshikawa; Amirul Islam; Qumrul Huda; Hiromu Takemura; Tetsuya Matsumoto; Shigeki Nakamura

doi:10.3389/fmicb.2020.612020

Characteristics of Carbapenemase-Producing Klebsiella pneumoniae Isolated in the Intensive Care Unit of the Largest Tertiary Hospital in Bangladesh

Front Microbiol. 2021 Jan 15:11:612020. doi: 10.3389/fmicb.2020.612020. eCollection 2020.

Authors

Takashi Okanda^{1

2

3}, Anwarul Haque³, Takuro Koshikawa², Amirul Islam⁴, Qumrul Huda⁴, Hiromu Takemura², Tetsuya Matsumoto³, Shigeki Nakamura¹

Affiliations

¹ Department of Microbiology, Tokyo Medical University, Tokyo, Japan.
² Department of Microbiology, St. Marianna University School of Medicine, Kawasaki, Japan.
³ Department of Infectious Diseases, International University of Health and Welfare, Narita, Japan.
⁴ Department of Anesthesia, Analgesia and Intensive Care Medicine, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh.

Abstract

For addressing the issue of antimicrobial drug resistance in developing countries, it is important to investigate the characteristics of carbapenemase-producing organisms. We aimed to genetically characterize a carbapenemase-producing Klebsiella pneumoniae (CPKP) isolated in the intensive care unit of a tertiary hospital in Bangladesh. The number of CPKP isolates were 43/145 (30%), of which pandrug-resistant (PDR) strains were 14%. These carbapenemases were New Delhi metallo-beta-lactamase (NDM)-1 (53%), NDM-5 (14%), oxacillinase (OXA)-181 (12%), OXA-232 (10%), NDM-5 + OXA-181 (5%), and NDM-5 + OXA-232 (2%). Many CPKP isolates harbored a variety of resistance genes, and the prevalence of 16S rRNA methyltransferase was particularly high (91%). The 43 CPKP isolates were classified into 14 different sequence types (STs), and the common STs were ST34 (26%), ST147 (16%), ST11 (9%), ST14 (9%), ST25 (7%), and ST231 (7%). In this study, PDR strains were of three types, ST147, ST231, and ST14, and their PDR rates were 57, 33, and 25%, respectively. The spread of the antimicrobial drug resistance of CPKP in Bangladesh was identified. In particular, the emergence of PDR is problem, and there may be its spread as a superbug of antimicrobial treatment.

Keywords: NDM-1; NDM-5; OXA-181; OXA-232; PDR; ST147.