Potential mechanisms underlying the ameliorative effect of Lactobacillus paracasei FZU103 on the lipid metabolism in hyperlipidemic mice fed a high-fat diet

Xu-Cong Lv; Min Chen; Zi-Rui Huang; Wei-Ling Guo; Lian-Zhong Ai; Wei-Dong Bai; Xiao-Dan Yu; Ying-Li Liu; Ping-Fan Rao; Li Ni

doi:10.1016/j.foodres.2020.109956

Potential mechanisms underlying the ameliorative effect of Lactobacillus paracasei FZU103 on the lipid metabolism in hyperlipidemic mice fed a high-fat diet

Food Res Int. 2021 Jan:139:109956. doi: 10.1016/j.foodres.2020.109956. Epub 2020 Dec 8.

Authors

Xu-Cong Lv¹, Min Chen², Zi-Rui Huang², Wei-Ling Guo³, Lian-Zhong Ai⁴, Wei-Dong Bai⁵, Xiao-Dan Yu², Ying-Li Liu⁶, Ping-Fan Rao⁷, Li Ni⁷

Affiliations

¹ Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: xucong1154@163.com.
² College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
³ Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China; College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China. Electronic address: 609969791@qq.com.
⁴ Shanghai Engineering Research Center of Food Microbiology, School of Medical Instruments and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
⁵ College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong 510225, China.
⁶ Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China; China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing Technology and Business University (BTBU), Beijing 100048, China. Electronic address: liuyingli@th.btbu.edu.cn.
⁷ Institute of Food Science and Technology, College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350108, China.

PMID: 33509508
DOI: 10.1016/j.foodres.2020.109956

Abstract

Lactobacillus paracasei FZU103, a probiotic previously isolated from the traditional brewing process of Hongqu rice wine, may have the beneficial effect of improving the disorder of lipid metabolism. This study aimed to determine the beneficial effects of L. paracasei FZU103 on improving hepatic lipid accumulation associated with hyperlipidemia. Results indicated that L. paracasei FZU103 intervention significantly inhibited the abnormal growth of body weight and epididymal white adipose tissue (eWAT), prevented the hypertrophy of epididymal adipocytes, ameliorated the biochemical parameters of serum and liver related to lipid metabolism in HFD-fed mice. Histological analysis also showed that the excessive accumulation oflipid dropletsin the livers induced by HFD-feeding was greatly alleviated by L. paracasei FZU103 intervention. In addition, L. paracasei FZU103 also promoted the excretion of bile acids (BAs) through feces. Metagenomic analysis revealed that oral supplementation with L. paracasei FZU103 significantly increased the relative abundance of Ruminococcus, Alistipes, Pseudoflavonifractor and Helicobacter, but decreased the levels of Blautia, Staphylococcos and Tannerella in HFD-fed mice. The relationships between lipid metabolic parameters and intestinal microbial phylotypes were also revealed by correlation heatmap and network. Furthermore, ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS)-based liver metabolomics demonstrated that L. paracasei FZU103 had a significant regulatory effect on the metabolic pathways of glycerophospholipid metabolism, fatty acid degradation, fatty acid elongation, unsaturated fatty acids biosynthesis, riboflavin metabolism, glycerolipid metabolism, primary bile acid biosynthesis, arachidonic acid metabolism, etc. Additionally, L. paracasei FZU103 intervention regulated expression of hepatic genes involved in lipid metabolism and bile acid homeostasis, and promoted fecal excretion of intestinal BAs. These findings present new evidence supporting that L. paracasei FZU103 has the potential to improve lipid metabolism, and could be used as a potential functional food for the prevention of hyperlipidemia.

Keywords: Hyperlipidemia; Intestinal microbiota; Lactobacillus paracasei FZU103; Lipid metabolism; UPLC-QTOF/MS-based liver metabolomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Diet, High-Fat
Gastrointestinal Microbiome*
Hyperlipidemias* / prevention & control
Lacticaseibacillus paracasei*
Lipid Metabolism
Mice