Comparative evaluation of chemical composition and biological activities of tropical fruits consumed in Manaus, central Amazonia, Brazil

Jéssica V Faria; Iris H Valido; Weider H P Paz; Felipe M A da Silva; Afonso D L de Souza; Leonard R D Acho; Emerson S Lima; Ana Paula A Boleti; Jane V N Marinho; Marcos J Salvador; Edson L Dos Santos; Patrícia K Soares; Montserrat López-Mesas; Jair M F Maia; Hector H F Koolen; Giovana A Bataglion

doi:10.1016/j.foodres.2020.109836

Comparative evaluation of chemical composition and biological activities of tropical fruits consumed in Manaus, central Amazonia, Brazil

Food Res Int. 2021 Jan:139:109836. doi: 10.1016/j.foodres.2020.109836. Epub 2020 Oct 26.

Authors

Jéssica V Faria¹, Iris H Valido², Weider H P Paz³, Felipe M A da Silva⁴, Afonso D L de Souza⁴, Leonard R D Acho⁵, Emerson S Lima⁵, Ana Paula A Boleti⁶, Jane V N Marinho⁷, Marcos J Salvador⁷, Edson L Dos Santos⁶, Patrícia K Soares⁸, Montserrat López-Mesas⁹, Jair M F Maia¹⁰, Hector H F Koolen¹¹, Giovana A Bataglion¹²

Affiliations

¹ Grupo de Pesquisas em Metabolômica e Espectrometria de Massas, Universidade do Estado do Amazonas, 690065-130 Manaus, Brazil.
² Grupo de Pesquisas em Metabolômica e Espectrometria de Massas, Universidade do Estado do Amazonas, 690065-130 Manaus, Brazil; Centre Grup de Técniques de Separació en Química (GTS), Departament de Química, Universitat Autònoma de Barcelona, Facultat de Ciències, Edifici CN, 08193 Bellaterra, Barcelona, Spain.
³ Grupo de Pesquisas em Metabolômica e Espectrometria de Massas, Universidade do Estado do Amazonas, 690065-130 Manaus, Brazil; Departamento de Química, Universidade Federal do Amazonas, 69080-900 Manaus, Brazil.
⁴ Departamento de Química, Universidade Federal do Amazonas, 69080-900 Manaus, Brazil.
⁵ Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas, 69077-000 Manaus, Brazil.
⁶ Faculdade de Ciências Biológicas e Ambientais, Universidade Federal de Grande Dourados, 79825-900 Dourados, Brazil.
⁷ Departamento de Biologia Vegetal, Universidade Estadual de Campinas, 13083-970 Campinas, Brazil.
⁸ Escola de Ciências e Tecnologia, Universidade Federal do Rio Grande do Norte, 59078-970 Natal, Brazil.
⁹ Centre Grup de Técniques de Separació en Química (GTS), Departament de Química, Universitat Autònoma de Barcelona, Facultat de Ciències, Edifici CN, 08193 Bellaterra, Barcelona, Spain.
¹⁰ Laboratório de Ecologia, Universidade do Estado do Amazonas, 69050-010 Manaus, Brazil.
¹¹ Grupo de Pesquisas em Metabolômica e Espectrometria de Massas, Universidade do Estado do Amazonas, 690065-130 Manaus, Brazil. Electronic address: hkoolen@uea.edu.br.
¹² Grupo de Pesquisas em Metabolômica e Espectrometria de Massas, Universidade do Estado do Amazonas, 690065-130 Manaus, Brazil; Departamento de Química, Universidade Federal do Amazonas, 69080-900 Manaus, Brazil. Electronic address: giovanabataglion@ufam.edu.br.

PMID: 33509461
DOI: 10.1016/j.foodres.2020.109836

Abstract

Fruits are widely recognized as sources of biologically active metabolites, such as antioxidant compounds. In this context, fruits commonly consumed in the central Amazonia, especially in its biggest metropolis (Manaus - AM/Brazil), are attractive as potential sources of antioxidant compounds related to biological activities. Most of such fruits are still poorly studied and/or remain unknown outside the Amazon region. Therefore, this study aims to investigate nine fruits (abiu, cubiu, biribá, breadfruit, genipap, peach palm, murici, soursop, and umari) regarding their chemical composition (fixed and volatile), reducing capacity, antioxidant activity, enzyme inhibition, and cytotoxicity. Determination of small organic acids, hydroxycinnamic acids, flavan-3-ols and flavonoid aglycones was done by HPLC-MS/MS, whereas determination of volatile organic compounds (VOCs) was done by HS-SPME/GC-MS. Reducing capacity was determined by the Folin-Ciocalteu method, and antioxidant activities were evaluated by DPPH, ABTS, and H-ORAC_FL assays. In vitro activities regarding inhibition of enzymes were tested for α-glucosidase, lipase, and α-amylase, and anti-glycation activities were evaluated for methylglyoxal and fructose. Cytotoxicity of fruit extracts was evaluated by cell viability of human fibroblast cell line (MRC-5). A total of 16 antioxidant compounds and 139 VOCs were determined, whose profiles were unique for each studied fruit. Total phenolic contents as well as antioxidant activities found herein were similar or even higher than those reported for several traditional fruits. Some of fruit extracts were able to inhibit α-glucosidase and glycation in methylglyoxal and fructose models, whereas none of them was active for lipase and α-amylase. All of the fruit extracts showed to be non-cytotoxic to MRC-5 cell line.

Keywords: Amazon; Annona muricata; Bactris gasipaes; Byrsonima crassifolia; Genipa Americana; Pouteria caimito; Solanum sessiliflorum; Volatile organic compounds.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antioxidants / pharmacology
Brazil
Fruit*
Humans
Malpighiaceae*
Tandem Mass Spectrometry

Substances

Antioxidants