Bulges in left-handed G-quadruplexes

Poulomi Das; Khac Huy Ngo; Fernaldo Richtia Winnerdy; Arijit Maity; Blaž Bakalar; Yves Mechulam; Emmanuelle Schmitt; Anh Tuân Phan

doi:10.1093/nar/gkaa1259

Bulges in left-handed G-quadruplexes

Nucleic Acids Res. 2021 Feb 22;49(3):1724-1736. doi: 10.1093/nar/gkaa1259.

Authors

Poulomi Das¹, Khac Huy Ngo¹, Fernaldo Richtia Winnerdy¹, Arijit Maity¹, Blaž Bakalar¹, Yves Mechulam², Emmanuelle Schmitt², Anh Tuân Phan^{1

3}

Affiliations

¹ School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Singapore.
² Laboratoire de Biologie Structurale de la Cellule (BIOC), Ecole Polytechnique, CNRS-UMR7654, Institut Polytechnique de Paris, Palaiseau 91128, France.
³ NTU Institute of Structural Biology, Nanyang Technological University, Singapore 636921, Singapore.

Abstract

G-quadruplex (G4) DNA structures with a left-handed backbone progression have unique and conserved structural features. Studies on sequence dependency of the structures revealed the prerequisites and some minimal motifs required for left-handed G4 formation. To extend the boundaries, we explore the adaptability of left-handed G4s towards the existence of bulges. Here we present two X-ray crystal structures and an NMR solution structure of left-handed G4s accommodating one, two and three bulges. Bulges in left-handed G4s show distinct characteristics as compared to those in right-handed G4s. The elucidation of intricate structural details will help in understanding the possible roles and limitations of these unique structures.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallography, X-Ray
DNA / chemistry*
G-Quadruplexes*
Models, Molecular
Nuclear Magnetic Resonance, Biomolecular
Nucleotide Motifs
Sugars / chemistry

Substances

Sugars
DNA