Cisplatin prevents breast cancer metastasis through blocking early EMT and retards cancer growth together with paclitaxel

Haitao Wang; Sen Guo; Seung-Jin Kim; Fangyuan Shao; Joshua Wing Kei Ho; Kuan Un Wong; Zhengqiang Miao; Dapeng Hao; Ming Zhao; Jun Xu; Jianming Zeng; Koon Ho Wong; Lijun Di; Ada Hang-Heng Wong; Xiaoling Xu; Chu-Xia Deng

doi:10.7150/thno.46460

Cisplatin prevents breast cancer metastasis through blocking early EMT and retards cancer growth together with paclitaxel

Theranostics. 2021 Jan 1;11(5):2442-2459. doi: 10.7150/thno.46460. eCollection 2021.

Authors

Haitao Wang^{1

2}, Sen Guo^{1

2}, Seung-Jin Kim³, Fangyuan Shao^{1

2}, Joshua Wing Kei Ho⁴, Kuan Un Wong^{1

2}, Zhengqiang Miao^{1

2}, Dapeng Hao^{1

2}, Ming Zhao^{1

2}, Jun Xu^{1

2}, Jianming Zeng^{1

2}, Koon Ho Wong^{1

5}, Lijun Di^{1

2}, Ada Hang-Heng Wong^{1

2}, Xiaoling Xu^{1

2}, Chu-Xia Deng^{1

2}

Affiliations

¹ Cancer Center, Faculty of Health Sciences, University of Macau, Macau SAR, China.
² Center for Precision Medicine Research and Training, University of Macau, Macau SAR, China.
³ Genetics of Development and Disease Branch, 10/9N105, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892, USA.
⁴ School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR, China.
⁵ Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau SAR, China.

Abstract

Cancer growth is usually accompanied by metastasis which kills most cancer patients. Here we aim to study the effect of cisplatin at different doses on breast cancer growth and metastasis. Methods: We used cisplatin to treat breast cancer cells, then detected the migration of cells and the changes of epithelial-mesenchymal transition (EMT) markers by migration assay, Western blot, and immunofluorescent staining. Next, we analyzed the changes of RNA expression of genes by RNA-seq and confirmed the binding of activating transcription factor 3 (ATF3) to cytoskeleton related genes by ChIP-seq. Thereafter, we combined cisplatin and paclitaxel in a neoadjuvant setting to treat xenograft mouse models. Furthermore, we analyzed the association of disease prognosis with cytoskeletal genes and ATF3 by clinical data analysis. Results: When administered at a higher dose (6 mg/kg), cisplatin inhibits both cancer growth and metastasis, yet with strong side effects, whereas a lower dose (2 mg/kg) cisplatin blocks cancer metastasis without obvious killing effects. Cisplatin inhibits cancer metastasis through blocking early steps of EMT. It antagonizes transforming growth factor beta (TGFβ) signaling through suppressing transcription of many genes involved in cytoskeleton reorganization and filopodia formation which occur early in EMT and are responsible for cancer metastasis. Mechanistically, TGFβ and fibronectin-1 (FN1) constitute a positive reciprocal regulation loop that is critical for activating TGFβ/SMAD3 signaling, which is repressed by cisplatin induced expression of ATF3. Furthermore, neoadjuvant administration of cisplatin at 2 mg/kg in conjunction with paclitaxel inhibits cancer growth and blocks metastasis without causing obvious side effects by inhibiting colonization of cancer cells in the target organs. Conclusion: Thus, cisplatin prevents breast cancer metastasis through blocking early EMT, and the combination of cisplatin and paclitaxel represents a promising therapy for killing breast cancer and blocking tumor metastasis.

Keywords: TGFβ; cisplatin; metastasis; neoadjuvant therapy; paclitaxel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Combined Chemotherapy Protocols / pharmacology*
Apoptosis
Breast Neoplasms / drug therapy*
Breast Neoplasms / metabolism
Breast Neoplasms / pathology
Cell Movement*
Cell Proliferation
Cisplatin / administration & dosage
Epithelial-Mesenchymal Transition*
Female
Humans
Lung Neoplasms / drug therapy*
Lung Neoplasms / metabolism
Lung Neoplasms / secondary
Mice
Mice, Nude
Paclitaxel / administration & dosage
Signal Transduction
Tumor Cells, Cultured
Xenograft Model Antitumor Assays

Substances

Paclitaxel
Cisplatin