Enhanced anticancer potency of hydroxytyrosol and curcumin by PLGA-PAA nano-encapsulation on PANC-1 pancreatic cancer cell line

Mahdieh Farhoudi Sefidan Jadid; Behrouz Shademan; Reza Chavoshi; Nasrin Seyyedsani; Elnaz Aghaei; Elham Taheri; Pouya Goleij; Saba Hajazimian; Vahidreza Karamad; Javad Behroozi; Mehrdad Nasrollahzadeh Sabet; Alireza Isazadeh; Behzad Baradaran

doi:10.1002/tox.23103

Enhanced anticancer potency of hydroxytyrosol and curcumin by PLGA-PAA nano-encapsulation on PANC-1 pancreatic cancer cell line

Environ Toxicol. 2021 Jun;36(6):1043-1051. doi: 10.1002/tox.23103. Epub 2021 Jan 26.

Affiliations

¹ Department of Genetics, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
² Department of Medical Biology, Faculty of Medicine, Ege University, Izmir, Turkey.
³ Department of Genetics, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran.
⁴ Department of Pharmaceutical Biotechnology, Tabriz University of Medical Sciences, Tabriz, Iran.
⁵ Department of Genetics, Faculty of Biology, Sana Institute of Higher Education, Sari, Iran.
⁶ Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
⁷ Department of Genetics and Biotechnology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran.

PMID: 33496383
DOI: 10.1002/tox.23103

Abstract

Many chemotherapeutic regimens have been investigated for advanced unresectable and metastatic pancreatic cancer (PC), but with only minimal improvement in survival and prognosis. Here, we investigated anti-cancer function of free and nano-encapsulated hydroxytyrosol (Hyd) and curcumin (Cur), and its combinations (Hyd-Cur) on PANC-1 cell line. The poly lactide-co-glycolide-co-polyacrylic acid (PLGA-co-PAA) nano-encapsulated Hyd and Cur were synthesized, and MTT assay was performed to evaluate cytotoxic effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur. Effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur were evaluated on viability, migration, morphological alterations, colony formation, and apoptosis on PANC-1 cells. We observed that free and nano-encapsulated Hyd, Cur, and Hyd-Cur significantly increased apoptosis rates as well as significantly decreased viability, migration, and colony formation in PANC-1 cells. According to our results, Hyd-Cur combination and nano-encapsulation therapy exerts more profound apoptotic and anti-proliferative effects on PANC-1 cells than free Hyd or Hyd monotherapy.

Keywords: PLGA-PAA copolymer; apoptosis; curcumin; hydroxytyrosol; pancreatic cancer.

MeSH terms

Antineoplastic Agents* / pharmacology
Cell Line
Cell Line, Tumor
Curcumin* / pharmacology
Humans
Nanoparticles* / toxicity
Pancreatic Neoplasms* / drug therapy
Phenylethyl Alcohol* / analogs & derivatives
Phenylethyl Alcohol* / pharmacology

Substances

Antineoplastic Agents
3,4-dihydroxyphenylethanol
Curcumin
Phenylethyl Alcohol