Washingtonia filifera seed extracts inhibit the islet amyloid polypeptide fibrils formations and α-amylase and α-glucosidase activity

Sonia Floris; Antonella Fais; Rosaria Medda; Francesca Pintus; Alessandra Piras; Amit Kumar; Piotr Marek Kuś; Gunilla Torstensdotter Westermark; Benedetta Era

doi:10.1080/14756366.2021.1874945

Washingtonia filifera seed extracts inhibit the islet amyloid polypeptide fibrils formations and α-amylase and α-glucosidase activity

J Enzyme Inhib Med Chem. 2021 Dec;36(1):517-524. doi: 10.1080/14756366.2021.1874945.

Authors

Sonia Floris¹, Antonella Fais¹, Rosaria Medda¹, Francesca Pintus¹, Alessandra Piras², Amit Kumar³, Piotr Marek Kuś⁴, Gunilla Torstensdotter Westermark⁵, Benedetta Era¹

Affiliations

¹ Department of Life and Environmental Sciences, University of Cagliari, Cagliari, Italy.
² Department of Chemical and Geological Sciences, University of Cagliari, Cagliari, Italy.
³ Department of Electrical and Electronic Engineering, University of Cagliari, Cagliari, Italy.
⁴ Department of Pharmacognosy and Herbal Medicines, Wroclaw Medical University, Wrocław, Poland.
⁵ Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden.

Abstract

Washingtonia filifera seeds have revealed to possess antioxidant properties, butyrylcholinesterase and xanthine oxidase inhibition activities. The literature has indicated a relationship between Alzheimer's disease (AD) and type-2 diabetes (T2D). Keeping this in mind, we have now evaluated the inhibitory properties of W. filifera seed extracts on α-amylase, α-glucosidase enzyme activity and the Islet Amyloid Polypeptide (IAPP) fibrils formation. Three extracts from seeds of W. filifera were evaluated for their enzyme inhibitory effect and IC₅₀ values were calculated for all the extracts. The inhibition mode was investigated by Lineweaver-Burk plot analysis and the inhibition of IAPP aggregate formation was monitored. W. filifera methanol seed extract appears as the most potent inhibitor of α-amylase, α-glucosidase, and for the IAPP fibril formation. Current findings indicate new potential of this extract that could be used for the identification or development of novel potential agents for T2D and AD.

Keywords: Washingtonia filifera; enzyme inhibition; islet amyloid polypeptide; α-amylase; α-glucosidase.

MeSH terms

Arecaceae / chemistry*
Dose-Response Relationship, Drug
Glycoside Hydrolase Inhibitors / chemistry
Glycoside Hydrolase Inhibitors / isolation & purification
Glycoside Hydrolase Inhibitors / pharmacology*
Humans
Islet Amyloid Polypeptide / antagonists & inhibitors*
Islet Amyloid Polypeptide / metabolism
Molecular Docking Simulation
Molecular Structure
Plant Extracts / chemistry
Plant Extracts / isolation & purification
Plant Extracts / pharmacology*
Seeds / chemistry
Structure-Activity Relationship
alpha-Amylases / antagonists & inhibitors*
alpha-Amylases / metabolism
alpha-Glucosidases / metabolism*

Substances

Glycoside Hydrolase Inhibitors
Islet Amyloid Polypeptide
Plant Extracts
alpha-Amylases
alpha-Glucosidases

Grants and funding

This research was funded by the Novo Nordisk [grant number: NNF 180c0034256] and Swedish diabetes foundation [grant number: DIA 2017–296] to GTW. This work was partially supported by University of Cagliari.