Identification and Analyzation of Differentially Expressed Transcription Factors in Endometriosis

Shanshan Cong; Qiuyan Guo; Yan Cheng; Jianhua Gao; Liyuan Sun; Jing Wang; Han Wu; Tian Liang; Guangmei Zhang

doi:10.3389/fmolb.2020.614427

Identification and Analyzation of Differentially Expressed Transcription Factors in Endometriosis

Front Mol Biosci. 2021 Jan 7:7:614427. doi: 10.3389/fmolb.2020.614427. eCollection 2020.

Authors

Shanshan Cong¹, Qiuyan Guo¹, Yan Cheng¹, Jianhua Gao¹, Liyuan Sun¹, Jing Wang¹, Han Wu¹, Tian Liang¹, Guangmei Zhang¹

Affiliation

¹ Department of Gynecology, The First Affiliated Hospital, Harbin Medical University, Harbin, China.

Abstract

Background: Endometriosis is interpreted as the existence of endometrium outside the uterine cavity, such as ovaries, fallopian tubes and pelvic cavity. Dysmenorrhea, abnormal menstruation, infertility, and chronic pelvic pain are the primary symptoms of endometriosis. Although there are many theories about the origin of endometriosis, the exact factor of the disease has not been confirmed. Therefore, many other mechanisms are still worth exploring. Materials and Methods: The gene lists of the transcription factors (TFs) were selected from the intersections of three databases. The limma R package was used to analyze the differentially expressed genes (DEGs) of GSE6364 and GSE7305 and the DEGs intersected with the TFs to obtain the differentially expressed TFs (DETFs). Subsequently, one-way ANOVA and Student's t-test were used to analyze the expression of DETFs in different phases of the endometrium and the endometrium of the infertile and fertile females with endometriosis, respectively. Enrichment analysis and PPI network were performed to reveal the molecular mechanisms of endometriosis. Finally, the plotROC R package was used to evaluate the sensitivity and specificity of hub TFs for the diagnosis of endometriosis. Results: A total of 54 DETFs were screened out in endometriosis. The expression of up-regulated DETFs was gradually increased from the early secretory to the proliferative phase of the endometrium. Most up-regulated DETFs increased expression in the endometrium of infertile females. The pathways of DETFs were mainly enriched in stem cell differentiation, transcription activity, steroid hormone receptor activity and herpes simplex virus. Two hub TFs (RUNX2 and BATF) and two sub-networks were finally acquired from the PPI network. RUNX2 and BATF also had high diagnostic value in endometriosis. Conclusion: We discovered and analyzed 54 DETFs that were closely related to endometriosis, which would contribute to explore new mechanisms of endometriosis and search for new diagnostic markers and effective therapeutic targets.

Keywords: BATF; RUNX2; endometriosis; infertility; transcription factors.