Beneficial effects of Diplectria barbata (Wall. Ex C. B. Clarke) Franken et Roos extract on aging and antioxidants in vitro and in vivo

Youngeun Hong; Hyunji Lee; Quangdon Tran; Choinyam Bayarmunkh; Damdindorj Boldbaatar; So Hee Kwon; Jongsun Park; Jisoo Park

doi:10.1007/s43188-020-00064-z

Beneficial effects of Diplectria barbata (Wall. Ex C. B. Clarke) Franken et Roos extract on aging and antioxidants in vitro and in vivo

Toxicol Res. 2020 Oct 20;37(1):71-83. doi: 10.1007/s43188-020-00064-z. eCollection 2021 Jan.

Authors

Youngeun Hong^#^{1

2}, Hyunji Lee^#^{1

2}, Quangdon Tran^{1

2}, Choinyam Bayarmunkh^{3

4}, Damdindorj Boldbaatar^{3

4}, So Hee Kwon⁵, Jongsun Park^{1

2}, Jisoo Park^{1

2

6}

Affiliations

¹ Department of Pharmacology, Metabolic Syndrome and Cell Signaling Laboratory, Institute for Cancer Research, College of Medicine, Chungnam National University, Daejeon, 35015 South Korea.
² Department of Medical Science, College of Medicine, Chungnam National University, Daejeon, 35015 South Korea.
³ Department of Graduate Education, Graduate school, Mongolian National University of Medical Sciences, Ulaanbaatar, 14210 Mongolia.
⁴ Department of Physiology, Mongolian National University of Medical Sciences, Ulaanbaatar, 14210 Mongolia.
⁵ College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon, 21983 South Korea.
⁶ Department of Life Science, Hyehwa Liberal Arts College, Daejeon University, Daejeon, 34520 South Korea.

^# Contributed equally.

Abstract

The purpose of this study is to explore the effects of Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roons (DFR) on wound healing, antioxidant and aging in Normal Human Dermal Fibroblast cell (NHDF) cells and mouse skin models. We investigated the effects of the aging process in vitro and in vivo. DFRtreated NHDF cells showed a concentration-dependent increase in the expression of extracellular matrix (ECM) proteins (Collagen-2.5-fold increase at 50 μg/ml, Elastin-1.5-fold increase at 1μg/ml) as well as an increase in proteins related to cell survival, differentiation, and development, while expression of aging proteins such as matrix metalloproteinase 3 (MMP-3) was decreased (5-fold decrease at 50 μg/ml). DFR treatment also led to enhanced expression of antioxidant proteins such as nuclear factor erythroid 2-related factor 2 (10-fold increase at 50 μg/ml) and heme oxygenase 1 (1.5-fold increase at 25 μg/ml). To further investigate the antioxidative effects of DFR extracts, the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities were also evaluated. DFR extracts improved wound healing and resulted in increased expression of ECM proteins, while enzymes involved in collagen degradation, including MMP-3, were decreased in NHDF cells as well as in a mouse model. This study demonstrates the anti-aging, antioxidant, and wound healing properties of DFR extracts. Therefore, DFR extracts present may facilitate skin protection and care.

Keywords: Anti-aging; Anti-oxidant; Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roos; Extracts; NHDF cells; Wound healing.