Preparation of Spherical Nucleic Acid Nanoparticles Containing a Self-immolative Poly(carbamate) Core

Shione Fukumoto; Mami Kawade; Kazunori Kimura; Yoshitsugu Akiyama; Akihiko Kikuchi

doi:10.2116/analsci.20SCN06

Preparation of Spherical Nucleic Acid Nanoparticles Containing a Self-immolative Poly(carbamate) Core

Anal Sci. 2021 May 10;37(5):781-784. doi: 10.2116/analsci.20SCN06. Epub 2021 Jan 22.

Authors

Shione Fukumoto¹, Mami Kawade¹, Kazunori Kimura¹, Yoshitsugu Akiyama², Akihiko Kikuchi¹

Affiliations

¹ Department of Materials Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo, 125-8585, Japan.
² Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo, 125-8585, Japan. yoshitsugu.akiyama@rs.tus.ac.jp.

PMID: 33487596
DOI: 10.2116/analsci.20SCN06

Abstract

We prepared a novel spherical nucleic acid, containing a core structure of self-immolative poly(carbamate) (PC), with aminobenzyl alcohol as a repeating unit, by conjugating an end-activated PC derivative with an amine-terminated oligoDNA on a solid support for PC-oligoDNA. Dynamic light-scattering measurements revealed a hydrodynamic diameter of 107 nm with a narrow size distribution. A fluorescent monomer with aminobenzyl alcohol is available for PC-oligoDNA synthesis to enhance the fluorescence emission by a domino-like disassembly of PC in response to various external stimuli.

Keywords: DNA conjugation; Spherical nucleic acids; poly(carbamate); self-immolative polymer.

MeSH terms

Carbamates
Fluorescence
Nanoparticles*
Nucleic Acids*

Substances

Carbamates
Nucleic Acids