Genetic improvement of wheat early vigor promote weed-competitiveness under Mediterranean climate

Abstract

Chemical weed-control is the most effective practice for wheat, however, rapid evolution of herbicide-resistant weeds threat food-security and calls for integration of non-chemical practices. We hypothesis that integration of alternative GA-responsive dwarfing genes into elite wheat cultivars can promote early vigor and weed-competitiveness under Mediterranean climate. We develop near-isogenic lines of bread wheat cultivars with GAR dwarfing genes and evaluate them for early vigor and weed-competitiveness under various environmental and management conditions to identify promising NIL for weed-competitiveness and grain yield. While all seven NILs responded to external gibberellic acid application, they exhibited differences in early vigor. Greenhouse and field evaluations highlighted NIL OC1 (Rht8andRht12) as a promising line, with significant advantage in canopy early vigor over its parental. To facilitate accurate and continuous early vigor data collection, we applied non-destructive image-based phenotyping approaches which offers non-expensive and end-user friendly solution for selection. NIL OC1 was tested under different weed density level, infestation waves, and temperatures and highlight the complex genotypic × environmental × management interactions. Our findings demonstrate the potential of genetic modification of dwarfing genes as promising approach to improve weed-competitiveness, and serve as basis for future breeding efforts to support sustainable wheat production under semi-arid Mediterranean climate.

Keywords: Bread wheat; Early vigor; G × E × M interactions; Image-based phenotyping; Integrated weed management; Rht dwarfing genes.