Eosinophils are granulocytes involved in inflammatory processes related to type 2 immune responses as allergy and parasitic infestation. Eosinophils are scarce in homeostatic situations, comprising 3-6% of total granulocytes. In peripheral blood, they have a life span of 18-25 h. These cells are characterized by the presence of several types of granules that are very important because of their functions. Recently, we have described that these immune cells are able to release exosomes, which are nanovesicles involved in intercellular communication and implicated in development of several diseases such as cancer, cardiovascular diseases, and asthma. Here, we describe a technique for isolation of eosinophil-derived exosomes from human peripheral blood and for their utilization in cell functional assays.
Keywords: Cell functional assays; Eosinophils; Exosomes; Isolation.