NMR assignments of the macro domain from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Biomol NMR Assign. 2021 Apr;15(1):137-142. doi: 10.1007/s12104-020-09996-x. Epub 2021 Jan 24.

Abstract

SARS-CoV-2 is a novel pathogen causing pneumonia named COVID-19 and leading to a severe pandemic since the end of 2019. The genome of SARS-CoV-2 contains a macro domain that may play an important role in regulating ADP-ribosylation in host cells and initiating viral replication. Here, we report the 1H, 13C, and 15N resonance assignments of the SARS-CoV-2 macro domain. This work provides the ground for further structural deciphering and biophysical investigation in protein function and antiviral agent design.

Keywords: COVID-19; Macro domain; SARS-CoV-2; Viral protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon Isotopes
  • Genome, Viral
  • Hydrogen
  • Hydrogen-Ion Concentration
  • Magnetic Resonance Spectroscopy*
  • Nitrogen Isotopes
  • Protein Binding
  • Protein Domains
  • Protein Structure, Secondary
  • SARS-CoV-2 / chemistry*
  • Temperature
  • Viral Nonstructural Proteins / chemistry*
  • Viral Proteins / chemistry*

Substances

  • Carbon Isotopes
  • Nitrogen Isotopes
  • Nitrogen-15
  • Viral Nonstructural Proteins
  • Viral Proteins
  • Hydrogen
  • Carbon-13