Fractionation, structural characteristics and immunomodulatory activity of polysaccharide fractions from asparagus (Asparagus officinalis L.) skin

Nifei Wang; Gege Jia; Xufeng Wang; Yan Liu; Zhenjing Li; Honghui Bao; Qingbin Guo; Changlu Wang; Dongguang Xiao

doi:10.1016/j.carbpol.2020.117514

Fractionation, structural characteristics and immunomodulatory activity of polysaccharide fractions from asparagus (Asparagus officinalis L.) skin

Carbohydr Polym. 2021 Mar 15:256:117514. doi: 10.1016/j.carbpol.2020.117514. Epub 2020 Dec 16.

Authors

Nifei Wang¹, Gege Jia², Xufeng Wang³, Yan Liu⁴, Zhenjing Li⁵, Honghui Bao⁶, Qingbin Guo⁷, Changlu Wang⁸, Dongguang Xiao⁹

Affiliations

¹ State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, PR China; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, PR China. Electronic address: wangnifei@tust.edu.cn.
² State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, PR China. Electronic address: gegejia@mail.tust.edu.cn.
³ State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, PR China. Electronic address: wangxufeng@mail.tust.edu.cn.
⁴ State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, PR China. Electronic address: liuyan19946006@163.com.
⁵ State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, PR China. Electronic address: lizhenjing@tust.edu.cn.
⁶ School of Food Science and Technology, Hubei University of Arts and Science, Xiangyang, 441053, PR China. Electronic address: honghui_bao@163.com.
⁷ State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, PR China; Key Laboratory of Food Nutrition and Safety (Tianjin University of Science and Technology), Ministry of Education, Tianjin, 300457, PR China. Electronic address: guoqingbin008322@tust.edu.cn.
⁸ State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, PR China; Key Laboratory of Food Nutrition and Safety (Tianjin University of Science and Technology), Ministry of Education, Tianjin, 300457, PR China. Electronic address: clw123@tust.edu.cn.
⁹ College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, PR China. Electronic address: Xiao99@tust.edu.cn.

PMID: 33483035
DOI: 10.1016/j.carbpol.2020.117514

Abstract

The physicochemical properties, structural features and structure-immunomodulatory activity relationship of pectic polysaccharides from the white asparagus (Asparagus officinalis L.) skin were systematically studied. Using sequential ethanol precipitation, five sub-fractions namely WASP-40, WASP-50, WASP-60, WASP-70 and WASP-80 with distinct degree of esterification (DE) and molecular weight (Mw) were obtained. The Mw and DE values were decreased with the increase of the ethanol concentrations. Structurally, although 4-α-D-GalpA was the dominant sugar residue in all fractions, the molar ratios were decreased, whereas other sugar residues including arabinose- and mannose-based sugar residues overall increased with the increase of ethanol concentration. In addition, the effects of sub-fractions on the RAW 264.7 cells indicated that pectic polysaccharides with the higher DE value showed a stronger immunomodulatory activity. Moreover, the structure-activity relationship was also discussed in this study, which extends the value-added application of asparagus and its processing by-products.

Keywords: Immunomodulatory activity; Polysaccharide fractions; Structural characterization; Structure-immunomodulatory relationship; White asparagus skin.

MeSH terms

Animals
Arabinose / isolation & purification
Asparagus Plant / chemistry*
Carbohydrate Sequence
Cell Proliferation / drug effects
Chemical Fractionation / methods
Esters / chemistry
Gene Expression / drug effects*
Immunologic Factors / chemistry*
Immunologic Factors / isolation & purification
Immunologic Factors / pharmacology
Interleukin-10 / genetics
Interleukin-10 / immunology
Interleukin-6 / genetics
Interleukin-6 / immunology
Mannose / isolation & purification
Mice
Molecular Weight
Phagocytosis / drug effects*
Plant Extracts / chemistry
Polysaccharides / chemistry*
Polysaccharides / isolation & purification
Polysaccharides / pharmacology
RAW 264.7 Cells
Structure-Activity Relationship
Tumor Necrosis Factor-alpha / genetics
Tumor Necrosis Factor-alpha / immunology

Substances

Esters
IL10 protein, mouse
Immunologic Factors
Interleukin-6
Plant Extracts
Polysaccharides
Tumor Necrosis Factor-alpha
interleukin-6, mouse
Interleukin-10
Arabinose
Mannose